Effect of Multi-Walled Carbon Nanotubes on MUC5AC and MUC5B Expression in Airway Epithelial Cells.
10.3342/kjorl-hns.2015.58.8.552
- Author:
Ji Hoon AHN
1
;
Hyeong Geun KIM
;
Bo Hyeon SEO
;
Yoon Seok CHOI
;
Si Youn SONG
;
Chang Hoon BAE
;
Yong Dae KIM
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Human airway epithelial cells;
MUC5AC;
MUC5B;
Multi-walled carbon nanotubes
- MeSH:
Carbon*;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells*;
Humans;
Inflammation;
Mucins;
Nanostructures;
Nanotubes, Carbon*;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction;
Reverse Transcription;
RNA, Messenger
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2015;58(8):552-557
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Multi-walled carbon nanotubes (MWCNT) are one of the most commonly used nanomaterials to date. Recent studies have demonstrated that MWCNT increase immune response and allergic inflammation in airway epithelial cells. However, the effects of MWCNT on mucin in human airway epithelial cells have not been reported. Therefore, in the present study, the effect of MWCNT on MUC16, MUC5AC, and MUC5B expressions were investigated in human airway epithelial cells. SUBJECTS AND METHOD: In mucin-producing human NCI-H292 airway epithelial cells and primary cultures of normal nasal epithelial cells, the effects of MWCNT on MUC16, MUC5AC, and MUC5B expression were analyzed by reverse transcription polymerase chain reaction, real-time polymerase chain reaction, and enzyme-linked immunosorbent assay. RESULTS: In human NCI-H292 airway epithelial cells, MWCNT significantly induced the expression MUC5AC and MUC5B mRNA and the production of MUC5AC and MUC5B protein. However, MWCNT did not induce the expression of MUC16 mRNA. In the primary cultures of normal nasal epithelial cells, MWCNT also induced the expression of MUC5AC and MUC5B mRNA and the production of MUC5AC and MUC5B proteins. CONCLUSION: The results of this study demonstrate that MWCNT induces MUC5AC and MUC5B expression in human airway epithelial cells. These findings provide important information about the biological role of MWCNT on mucus-secretion in human airway epithelial cells.
