Effects of Ipriflavone on bone remodeling in the rat calvarial cell.
- Author:
Yong Seung LEE
1
;
Young Jun KIM
;
Ki Heon LEE
;
Hyeon Shik HWANG
Author Information
1. Department of Orthodontics, School of Dentistry, Chonnam National University, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Ipriflavone;
Calvarial cell;
Bone remodeling
- MeSH:
Animals;
Bone Remodeling*;
Bone Resorption;
Cell Differentiation;
Cell Proliferation;
Cells, Cultured;
Collagen Type I;
Extracellular Matrix;
Fetus;
Integrin-Binding Sialoprotein;
Osteocalcin;
Osteogenesis;
Rats*
- From:Korean Journal of Orthodontics
2005;35(4):275-285
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Ipriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone diazein, has been shown to inhibit bone resorption and perhaps stimulate bone formation. This study was performed to examine the effects of ipriflavone on the proliferation and bone remodeling in rat calvarial cells in vitro. The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured in BGJb media. The graded concentration of ipriflavone (10(-9)-10(-5) M) was administered into cultured cells. When the cell proliferation was estimated through the measurement of MTT assay, there was no increase in cellular proliferation of the rat calvarial cell at any ipriflavone concentration. The cellular activity was evaluated through the formation of mineralized nodules stained by alizarin red. The formation of mineralized nodules significantly increased at concentrations of 10(-8) M, 10(-7) M and 10(-6) M ipriflavone. Reverse transcription-polymerase chain reaction analyses (RT-PCR) were done at 7 and 14 days after culture to detect the expression of Bone Sialoprotein (BSP), Type I Collagen (COL I) and Osteocalcin (OCN). As a result, the expressions of BSP and COL I increased on the 7th day of culture and the expression of OCN increased on the 14th day of culture. These results indicate that ipriflavone facilitates the bone remodeling process by promoting rat calvarial cell differentiation and stimulating mineralization through increased expression of extracellular matrix genes, such as BSP, COL I and OCN.
