Indol-3-Carbinol Regulated Tight Junction Permeability and Associated-Protein Level and Suppressed Cell Invasion in Human Colon Cancer Cell Line, HT-29.
- Author:
Sung Ok KIM
1
;
Yung Hyun CHOI
;
Won Kyung CHOE
Author Information
1. Department of Pharmaceutical Science, University of Maryland, School of Pharmacy, Baltimore, MD 21201, USA.
- Publication Type:Original Article
- Keywords:
indol-3-carbinol;
TER;
claudin;
invasion
- MeSH:
Autolysis;
Cadherins;
Cell Line;
Cell Movement;
Claudin-1;
Claudin-5;
Collagen;
Colon;
Colonic Neoplasms;
Drug Combinations;
Glucosinolates;
HT29 Cells;
Humans;
Indoles;
Intercellular Junctions;
Laminin;
Neoplasm Metastasis;
Ovarian Neoplasms;
Permeability;
Proteins;
Proteoglycans;
RNA, Messenger;
Tight Junction Proteins;
Tight Junctions;
Vegetables;
Wound Healing
- From:The Korean Journal of Nutrition
2008;41(1):13-21
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To determine whether indol-3-carbinol (I3C, C9H9NO), an autolysis product of a glucosinolate and a glucobrassicin in vegetables, regulated tight junction proteins (TJ) and suppressed cell invasion in colon cancer cells,this experiment was performed. Our results indicate that I3C inhibit cell growth of HT-29 cells in a dose (0, 50, 100 micrometer) and time (0, 24 and 48 h) dependent manner. Using the wound healing and matrigel invasion study, respectively, I3C inhibits the cell motility and invasion of the ovarian cancer cell line. The TEER values were increased in HT-29 cells grown in transwells treated with I3C, reversely, paracellular permeability was decreased in those of condition. Claudin-1, claudin-5, ZO-1 and occuldin have been shown to be positively expressed in HT-29 coloncancer cells. I3C occurs concurrently with a significant decrease in the levels of those of proteins in HT-29 cells. But E-cadherin level in the HT-29 was increased by I3C. The reduction of claudin-1 and claudin-5 protein levels occurred post-transcriptionaly since their mRNA levels are no difference by I3C. Therefore, our results suggest that I3C may be expected to inhibit cancer metastasis and invasion by tighten the cell junction and restoring tight junction in colon cancer cell line, HT-29.
