Expression of Leukocyte-Type 12-Lipoxygenase in Murine Nasal Mucosa According to the Development.
- Author:
Hee Sun CHUN
1
;
Joo Heon YOON
;
Joo Hwan LEE
;
Yoon Jae LEE
;
Tae Joon PARK
;
Kyung Su KIM
Author Information
1. Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea. ydrhinol@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Arachidonate 12-lipoxygenase;
Nasal mucosa;
Development;
Immunohistochemistry
- MeSH:
Adult;
Alcian Blue;
Animals;
Arachidonate 12-Lipoxygenase*;
Epithelial Cells;
Goblet Cells;
Humans;
Immunohistochemistry;
Mice;
Nasal Mucosa*;
Olfactory Mucosa;
Respiratory Mucosa
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2005;48(1):40-45
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: We determined the localization of leukocyte-type 12-lipoxygenase (L-12-LO) in murine nasal mucosa and to investigate the expression of L-12-LO according to the development of murine nasal mucosa. MATERIALS AND METHOD: Immunohistochemical staining was done on the nasal mucosa of mice at gestational days 16, 17, 18, and mice at postnatal days 1, 3, 7, 14, and adult mice. Alcian blue (pH 2.5)-periodic acid Schiff staining on murine nasal mucosa was performed. RESULTS: In murine nasal respiratory mucosa, the expression of L-12-LO was noted in ciliated epithelial cells, basal cells, serous acini, and secretory ducts, but it was not found in the mucous acini and goblet cells. In olfactory mucosa, the expression of L-12-LO was noted in the olfactory receptor cells, supporting cells, and basal cells. The expression in respiratory mucosa according to the development was strongly noticed from the gestational day 16 through postnatal day 7. The expression in postnatal day 14 and adult mice was weaker than in the previous time point. The expression in olfactory mucosa showed no difference throughout the developmental stage. CONCLUSION: As a result of this study, we found the exact localization of L-12-LO in murine nasal mucosa, and we also found the different expression of L-12-LO between the respiratory and olfactory mucosa. This fact suggests the possible involvement of L-12-LO in the development of murine respiratory mucosa.
