Effect of Caffeic Acid Phenethyl Ester on Lipopolysaccharide-induced Murine Macrophage Activation.
10.4266/kjccm.2011.26.3.134
- Author:
Seong Heon LEE
1
;
Mei LI
;
Dae Wook LEE
;
Dong Yun LIM
;
Cheol Won JEONG
;
Sang Hyun KWAK
Author Information
1. Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, Gwangju, Korea. shkwak@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
caffeic acid phenethyl ester;
cytokines;
mitogen-activated protein kinases
- MeSH:
Caffeic Acids;
Cytokines;
Extracellular Signal-Regulated MAP Kinases;
Interleukins;
Macrophage Activation;
Macrophages;
Mitogen-Activated Protein Kinases;
Phenylethyl Alcohol;
Phosphorylation;
Phosphotransferases;
Propolis;
Tumor Necrosis Factor-alpha
- From:The Korean Journal of Critical Care Medicine
2011;26(3):134-138
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Caffeic acid phenethyl ester (CAPE) is an active component of propolis and is known to have anti-inflammatory properties. This study was performed to evaluate the effects of CAPE on lipopolysaccharide (LPS)-induced murine macrophage activation. METHODS: Raw 264.7 cells were incubated with varying concentrations of CAPE with or without LPS. The production of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta and macrophage inflammatory protein-2 (MIP-2) and activation of extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 were measured. RESULTS: CAPE inhibited the production of TNF-alpha, IL-1beta and MIP-2 and attenuated phosphorylation levels of ERK1/2 and p38, but not JNK in RAW264.7 cells stimulated with LPS. CONCLUSIONS: CAPE can attenuate LPS-induced macrophage responses and we suggest that these effects may play an important role in modulating macrophage-mediated inflammatory responses in vivo.
