Effects of Interleukin-1 on Cell Proliferation in UMR-106-01 Osteoblast-like cells.
- Author:
Beom Koo LEE
;
Byung Jik KIM
;
Jae Dam LEE
;
Geon Beom KIM
- Publication Type:Original Article
- Keywords:
Interleukin-1;
Osteoblast-like cells;
Proliferation
- MeSH:
Animals;
Bone Resorption;
Cell Proliferation*;
Collagen;
DNA;
Glycoproteins;
Hand;
Insulin;
Interleukin-1*;
Interleukins;
Osteoblasts;
Osteosarcoma;
Parathyroid Hormone;
Rats;
Thymidine
- From:The Journal of the Korean Orthopaedic Association
1999;34(1):165-172
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Interleukin 1(IL-1), a 17.5 KD glycoprotein, is known to be associated with local bone resorption. In the present study, we examined the effects of IL-1, compared with insulin and parathyroid hormone (PTH), on DNA, protein and collagen synthesis in UMR-106-01 rat osteoblastic osteosarcoma cells. When 200 units/mL IL-1 was administered to UMR-106-01 cells, [3H]-thymidine uptake increased to 119% of the untreated control. But when 10 nM insulin was added to the cells, [3H]- thymidine uptake increased to 130% and when 1 nM PTH was added, the uptake decreased to 89% of the control. On the other hand, protein and collagen synthesis, measured by [3H]-leucine and [3H]-proline incorporation respectively, were not affected by IL-1 administration compared to the other hormones. These results indicate that IL-1 effects osteoblast-like cells, stimulating DNA synthesis via a different mechanism to the well-known cell growth factor, insulin.
