CoPPIX Protects against TNBS Induced Colitis Through HO-1 Induction.
- Author:
JaeMin OH
1
;
JinOh KIM
;
Young Mi KWON
;
MinWook RHEU
;
YuRim KIM
;
KyoungSuk KIM
;
SeungTaeck PARK
;
JeongJoong KIM
;
MinKyu CHOI
;
YeunTai CHUNG
Author Information
1. Department of Anatomy, School of Medicine, Wonkwang Medical Science Institute, Wonkwang University, Korea. jmoh@wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
CoPPIX;
HO-1;
NF kappa B;
TNBS colitis;
Crohn`s disease
- MeSH:
Animals;
Cobalt;
Colitis*;
Colon;
Copper;
Heme Oxygenase-1;
Immunosuppression;
Inflammation;
Interleukin-1beta;
Mice;
Transcription Factors;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Anatomy
2004;37(5):459-466
- CountryRepublic of Korea
- Language:English
-
Abstract:
Crohn`s disease is a severe chronic inflammation that is treated mainly by immunosuppression, which often has serious side effects. There is a need to develop new drugs for treating this disease that have few side effects. Heme oxygenase-1 (HO-1) has immunosuppressive properties, but the mechanism of its anti-inflammatory actions is unclear. We investigated the protective effects of HO-1 on trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. An HO-1 inducer, cobalt protoporphyrin IX (CoPPIX), dramatically improved the clinical and histopathological symptoms in TNBS-induced colitis. CoPPIX suppressed tumor necrosis factor-alpha and interleukin-1beta expression and down-regulated the nuclear transcription factor kappa B activity caused by TNBS. The vehicle copper protoporphyrin IX (CuPPIX) failed to duplicate the protective effects seen with CoPPIX. Moreover, an inhibitor of HO-1 activity-zinc protoporphyrin IX-reversed the protective effects of CoPPIX on TNBS-induced colitis. In conclusion CoPPIX protects against TNBS-induced colonic damage by inducing HO-1, which might be an important target in the treatment of Crohn`s disease.
