Roles of Stem Cell Factor and c-kit Receptor in the Development of Cerebellar GABAergic Neurons.
- Author:
You Jin WON
1
;
Jong Hwan LEE
;
Jin Ok IM
;
Seung Yong YUN
;
Seung Jun HWANG
;
Donghou KIM
;
Hea Nam HONG
Author Information
1. Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, 388-1 Pungnap-dong, Songpa-gu, Seoul 138-736, South Korea. hnhong@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Stem cell factor;
c-kit receptor;
Cerebellar inhibitory interneurons;
Purkinje cells;
Postnatal development;
GAD65/67
- MeSH:
Animals;
Calbindins;
Cerebellum;
Culture Media, Serum-Free;
GABAergic Neurons*;
Glutamate Decarboxylase;
Interneurons;
Mice;
Neurites;
Neuritis;
Protein-Tyrosine Kinases;
Proto-Oncogene Proteins c-kit*;
Purkinje Cells;
Stem Cell Factor*;
Stem Cells*
- From:Korean Journal of Anatomy
2004;37(5):481-490
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Evidence that Stem cell factor (SCF) and c-Kit receptor tyrosine kinase are expressed in the cerebellum during postnatal development, suggests a possible contribution of the SCF/Kit signaling pathway in the cerebellar development. In the present study, we prepared cerebellar cultures from C57Bl/6J mouse at postnatal day 1and 7 to investigate the role of c-kit receptor and SCF in regulation of growth and differentiation in the postnatal cerebellar GABAergic cells. SCF increased the number of survival cerebellar cells and density of glutamic acid decarboxylase 65/67 (GAD65/67) and calbindin D-28K expression in the immunoblot analysis. SCF also improved the neurite extension of the interneuron neuritis and dendritogenesis of Purkinje cells. Treatment with c-Kit antibody accelerated cellular loss in serum-free media and decreased the growth ability and dendritogenesis of Purkinje cells and cerebellar inhibitory interneurons. Our data suggest that SCF and c-kit receptor are required for the normal growth of postnatal cerebellum and a possible involvement of functional regulation through the SCF/c-kit receptor pathways in the postnatal cerebellar development.
