Genetic Susceptibilities of Cytochrome P4501A1 and Glutathione S-transferase M1 to the Risk for Korean Head and Neck Squamous Cell Carcinoma Patients.
- Author:
Chang Sik SHIN
1
;
Kyung Sung AHN
;
Kyung TAE
;
Hyung Seok LEE
;
Hyun Jun KIM
;
Gu KONG
Author Information
1. Department of Otolaryngology, College of Medicine, Hanyang University, Seoul, Korea. kytae@email.hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Head and neck cancer;
Cytochrome P450;
Glutathione S-transferase;
Genetic polymorphism
- MeSH:
Carcinogens;
Carcinoma, Squamous Cell*;
Cytochrome P-450 CYP1A1;
Cytochrome P-450 Enzyme System;
Cytochromes*;
Genetic Predisposition to Disease*;
Genotype;
Glutathione Transferase*;
Glutathione*;
Head and Neck Neoplasms;
Head*;
Humans;
Larynx;
Neck*;
Pharynx;
Polymerase Chain Reaction;
Polymorphism, Genetic
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1999;42(2):202-208
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Individual genetic susceptibilities to chemical carcinogens have been recognized as a major important host factors in human cancers. The cytochrome P450 family (CYPs) and glutathione-S-transferase (GST) have been reported to be associated with risks to the smoking-related human cancers. The purpose of this study is to determine the frequencies of the genotypes of CYP1A1 and GSTM1 genes in healthy control of Koreans and to identify the high-risk genotypes of these metabolic genes in head and neck cancer patients. MAERIALS AND METHODS: Ninety-six healthy controls and 93 head and neck squamous cell carcinoma patients are analysed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: The distributions of CYP1A1 and GSTM1 in healthy controls according to the MspI site and absence or presence of PCR products were as following: m1/m1:m1/m2:m2/m2=39.6%:47.9%:12.5%, GSTM1 (-):GSTM1 (+)=45%:55%. GSTM1 (-) type and CYP1A1 m2/m2 types were more frequent in cancer patients than healthy controls. Among the combined genotypes of CYP1A1 and GSTM1 genes, the relative risk of CYP1A1 m1/m2, GSTM1 (-) genotypes was 2.13 times of relative odds' ratio in head and neck cancer patients. According to the tumor location, CYP1A1 m2/m2, GSTM1 (-) genotypes of larynx and CYP1A1 m1/m2, GSTM1 (-) genotypes of oral and pharynx were the highest risk groups to cancers in their locations. CONCLUSION: These results suggest that the genetic polymorphisms of CYP1A1 and GSTM1 were an important major factor to determine the individual susceptibility to head and neck cancers in Korean. And these polymorphisms and cancers susceptibile genotypes of CYP1A1 and GSTM1 in Korean population are very unique in comparison with the other ethinics.
