PCR Detection of Mycobacterium Tuberculosis from Fine Needle Aspirate for the Diagnosis of Cervical Tuberculous Lymphadenitis.
- Author:
Chung Hwan BAEK
1
;
Sun Il KIM
;
Yang Sun CHO
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Sungkyunkwan University, College of Medicine, Samsung Medical Center, Seoul, Korea. chback@smc.samsung.co.kr
- Publication Type:Original Article
- Keywords:
Tuberculosis;
Lymph node;
Polymerase chain reaction;
Cytology
- MeSH:
Biopsy;
Biopsy, Fine-Needle;
Diagnosis*;
DNA;
Follow-Up Studies;
Humans;
Lymph Nodes;
Lymphatic Diseases;
Mycobacterium tuberculosis*;
Mycobacterium*;
Needles*;
Polymerase Chain Reaction*;
Sensitivity and Specificity;
Sequence Analysis, DNA;
Tuberculosis;
Tuberculosis, Lymph Node*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1999;42(2):209-214
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Despite its well-established usefulness in the diagnosis of cervical tuberculous lymphadenitis, fine needle aspiration cytology (FNAC) has limitations in its clinical applications, especially whenever the presence of acid-fast bacilli is not proven. Furthermore, fine needle aspirate is occasionally inadequate for the diagnosis, and the sensitivity and specificity of this technique for cervical tuberculous lymphadenitis have not been firmly established. We performed Mycobacterium tuberculosis (MTb) polymerase chain reaction (PCR) for mycobacterial DNA sequencing using the remainder of fine needle aspirates after the cytologic examination, and evaluated its diagnostic efficacy in clinical situations. MATERIALS & METHODS: Conventional diagnostic procedures including FNAC and MTb PCR were performed simultaneously in twenty-nine patients who had been suspected with cervical tuberculous lymphadenitis on their first visit. After several months of follow-up, the results of FNAC and MTb PCR were compared with the clinical outcomes in some cases. RESULTS: In 17 cases diagnosed clinically as cervical tuberculous lymphadenitis, MTb DNA was found by PCR in 13 cases (76.4%). Also, negative results were achieved in all 12 cases which revealed non-granulomatous lymphadenopathy. CONCLUSION: From these results, we conclude that MTb PCR using the remainder of aspirate for cytologic examination would be a very useful tool for the diagnosis of cervical tuberculous lymphadenitis, and its clinical application with FNAC could decrease the necessity of open biopsy.
