Up-regulation of Surfactant Protein-A in Chronic Sialadenitis.
- Author:
Il Ho PARK
1
;
Jeong Soo WOO
;
Jung Kyu LEE
;
Hyun Woo LIM
;
Soon Jae HWANG
;
Heung Man LEE
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea. lhman@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Surfactant protein A;
Sialadenitis
- MeSH:
Collectins;
Densitometry;
Epithelium;
Humans;
Pulmonary Surfactant-Associated Protein A;
RNA, Messenger;
Salivary Glands;
Sialadenitis*;
Up-Regulation*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2006;49(4):411-414
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Surfactant protein-A (SP-A) is a member of the collectin family, and plays an important role in the first-line airway defense. The purpose of study was to examine the expression of SP-A mRNA and protein in human salivary glands, and to investigate its up-regulation during inflammatory conditions of salivary glands. MATERIALS AND METHOD: Reverse transcription-polymerase chain reaction was performed on salivary gland tissues from ten patients with chronic sialadenitis and ten samples of normal salivary gland tissue. The expression levels of SP-A to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) transcripts were semi-quantified by densitometry. We also characterized the cellular localizations of SP-A protein immunohistochemically. RESULTS: SP-A mRNA and protein were detected in normal and chronic sialadenitis glands. The expression levels of SP-A mRNA in salivary glands with chronic sialadenitis was significantly increased as compared with normal salivary glands. Immunohistochemical staining revealed SP-A immunoreactivity in the ductal epithelia of normal salivary glands and chronic sialadenitis, and stronger immunoreactivity was observed in chronic sialadenitis tissues. CONCLUSION: SP-A is present in the human salivary gland epithelium and is up-regulated during chronic sialadenitis. These results suggest that salivary gland SP-A may play an important role in the innate host defense of human salivary glands.
