Relationship between Bone Morphological Microstructure and Inflammatory Markers in Growing Mice Fed a High Fat Diet.
10.4163/kjn.2011.44.6.481
- Author:
Mi Sung KIM
1
;
Hyun A LEE
;
Ok Jin KIM
;
Cheong Min SOHN
Author Information
1. Department of Food and Nutrition, Wonkwang University, Iksan 570-749, Korea. ccha@wku.ac.kr
- Publication Type:Original Article
- Keywords:
high fat diet;
inflammation markers;
bone morphological microstructure
- MeSH:
Animals;
Bone Density;
Diet;
Diet, High-Fat;
Glucose;
Humans;
Inflammation;
Interleukin-6;
Interleukins;
Leptin;
Male;
Mice;
Obesity;
Osteocalcin;
Osteogenesis;
Triglycerides
- From:The Korean Journal of Nutrition
2011;44(6):481-487
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Obesity not only reduces bone mineral density but also increases inflammatory markers. Therefore, we examined the change in inflammatory markers and morphological microstructure of the bones using a mouse model fed a high-fat diet. C57BL/6J 4-week-old male mice were divided into a control group (n = 6) and a experimental group (n = 6); the control group was provided with 10% Kcal fat diet, and the high-fat diet group was provided with 45% Kcal fat diet for 12 weeks using the free provision method. Blood was analyzed for inflammatory markers, and micro-computed tomography was used to measure the morphological microstructure of the femoral bone. The weight increases in the control group and high-fat diet group were 5.85 +/- 1.84 g and 16.06 +/- 5.64 g, respectively (p < 0.01), glucose was 115.00 +/- 16.88 mg/dL and 188.33 +/- 13.29 mg/dL (p < 0.01), and triglycerides were 65.00 +/- 6.19 mg/dL and 103.33 +/- 8.02 mg/dL (p < 0.05) respectively. Leptin and interleukin (IL)-6 were significantly higher in the high-fat diet group than that in the control group (p < 0.01). As a result of a biochemical index analysis of bone metabolism, osteocalcin tended to be lower in the high-fat diet group, whereas CTx was significantly higher in the high-fat diet group compared to that in the control group (p < 0.01). The thickness of the bony trabecula was significantly narrower in the high-fat diet group than that in the control group (p < 0.05), and the gap in the bony trabecula was significantly wider in the high-fat diet group than that in the control group (p < 0.05). IL-6 and the gap in the bone trabecula, which was a morphological microstructure of the bones, showed a positive correlation (p < 0.05). Taken together, inducing obesity through a high-fat diet in mice during the growth phase caused a change in bone microstructure and was correlated with the inflammation index. Accordingly, restriction of excessive fat intake may be needed to suppress the inflammatory reactions and promote normal bone formation.
