Expression of TGF -beta I and II Ligands and Receptors at Epiphyseal Plate and Fracture Callus.
- Author:
Kwan Hee LEE
;
Young In LEE
;
Kyu Chul CHO
;
In Suk OH
;
Joung Yoon LEE
;
Sung Jin KIM
- Publication Type:Original Article
- Keywords:
Transforming growth factor-beta;
Ligand;
Receptor;
Fracture callus;
Epiphyseal plate
- MeSH:
Animals;
Bony Callus*;
Cartilage;
Femur;
Growth Plate*;
Ligands*;
Periosteum;
Rats;
Receptors, Artificial;
Transforming Growth Factor beta;
Transforming Growth Factor beta1
- From:The Journal of the Korean Orthopaedic Association
1998;33(2):458-465
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To understand the expression of hoth TGF-beta l and II ligands and the receptors, artificial fracture was made on rat femur. Fracture callus and epiphyseul plate were stained immunohistochemically on 3rd. 7th, 14th, 21st, 42nd and 56th day after trauma. Polyclonal antibody was used to stain TGF-beta I and II ligands and receptors. At epiphyseal plate, both ligand and receptor were expressed from each cell in proliferating and maturing zone. But there was no difference between type I and II except expression time. TGF-beta II ligand and receptor were expressed earlier: they were expressed mostly by the cells at the zone of proliferating cartilage but TGF-beta1 ligand and receptor were expressed mostly hy the cells at zone of maturing cartilage. At fracture site, TGF-beta expression was observed from 3rd day after trauma and it reached its maximum intensity at 2 weeks. It decreased thereafter and disappeared at 6 weeks after trauma. In enchondral ossification area, TGF-beta expressing cells were scattered throughout the enchondral mass. In intramembranous ossification area, the ligands and receptors were expressed from the osteohlasts just heneath the periosteum. ln summary, TGF-beta ligands and receptors were expressed at epiphyseal plate and fracture callus. There was no difference between TGF-beta 1 and 2 expres.ion except the appearance time at epiphyseal plate. We could not draw any conclusion about ligand and rcceptor mechanism with this immunohistochemical staining.
