Ultrastructural Alterations Induced by 5-Fluorouracil or Mitomycin C on the Gastric Parietal Cells of Mouse.
- Author:
Jeong Sik KO
1
;
Byung Seop SHIN
;
E Tay AHN
;
Kyung Ho PARK
;
Jin Gook KIM
Author Information
1. Department of Anatomy, Soonchunhyang University College of Medicine, Cheonan, Korea. jeongsik@sch.ac.kr
- Publication Type:Original Article
- Keywords:
Parietal cell;
Gastric mucosa;
Ultrastructure;
5 -fluorouracil;
Mitomycin C
- MeSH:
Animals;
Citric Acid;
Cytoplasm;
Fluorouracil*;
Gastric Mucosa;
Glutaral;
Lysosomes;
Membranes;
Mice*;
Microvilli;
Mitomycin*;
Multivesicular Bodies;
Myelin Sheath;
Organelles;
Osmium Tetroxide;
Parietal Cells, Gastric*;
Phenobarbital;
Rabeprazole;
Stomach
- From:Korean Journal of Anatomy
2002;35(5):363-376
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This experiment was performed to evaluate the morphological responses of 5-fluorouracil or mitomycin C on the gastric parietal cells of mouse. 5 -fluorouracil (30 mg/kg) or mitomycin C (400 micro gram/kg) were injected subcutaneously every other day, and the animals were sacrificed at 4th day and 7th day following the first injection. Pieces of the tissue were taken from the stomach, prefixed with 2.5% glutaraldehyde -1.5% paraformaldehyde, followed by post-fixation with 1% osmium tetroxide. The ultrathin sections were stained with uranyl acetate and lead citrate. In both of the 5-fluorouracil or the mitomycin C treated groups, most parietal cells showed severely reduced luminal spaces of the intracellular canaliculi, since microvilli of intracellular canaliculi were very irregular shaped and nearly contacted with each other, and the cytoplasmic tubulovesicular membranes were disintegrated and indistinct. The changes in the 5-fluorouracil treated group were more indistinct than in those of the mitomycin C treated group. In the 5-fluorouracil treated group, balooning of the cytoplasm, focal cytolysis, myelin figures, lysosomes and multivesicular bodies in the parietal cells were observed more frequently than in those of the mitomycin C treated group. Above results suggest that the 5-fluorouracil or mitomycin C treated animals might suffer from reduced acid secretion of the parietal cell, since the collapsed lumen of the intracellular canaliculi, the disintegration of the tubulovesicular membranes, and the reduction of cell organelles in the parietal cells are occurred within a few days following injections. 5-fluorouracil was proved more harmful on the parietal cell than mitomycin C does.
