Change of Interstitial Cells of Cajal (ICC) and Intestinal Motility in Murine Small Bowel Obstruction.
- Author:
Jae Yeoul JUN
1
;
Choon Hae CHUNG
;
Ho Jin YOU
;
Kyung Hee KIM
;
Jang Man KIM
;
Kee Hune KIM
;
Do Young PARK
;
In Youb CHANG
Author Information
1. Department of Anatomy, College of Medicine, Chosun University, Korea. iyjang@mail.chosun.ac.kr
- Publication Type:Original Article
- Keywords:
c -Kit;
Interstitial cells of Cajal (ICC);
Slow wave;
Gastrointestinal motility;
Mouse
- MeSH:
Animals;
Enteric Nervous System;
Gastrointestinal Motility*;
Humans;
Hypertrophy;
Immunohistochemistry;
Interstitial Cells of Cajal*;
Intestinal Obstruction;
Intestine, Small;
Mice;
Muscle, Smooth
- From:Korean Journal of Anatomy
2002;35(5):387-396
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Interstitial cells of Cajal (ICC) are the pacemakers in gastrointestinal slow wave, and also transduce signal inputs from the enteric nervous system to smooth muscle. The abnormal motility corresponded to a lack or decreasing of ICC and a disruption of electrical slow waves. So we developed partial obstruction model in murine small intestine and investigated changes in the ICC networks and electrical activity in the obstructed bowel using c-kit immunohistochemistry and intracelluar electrophysiological techniques. Two weeks following the onset of a partial obstruction, the small intestine increased in diameter and muscular hypertrophy was developed oral to the obstruction site. ICC were absent or only weak at 1 ~25 mm oral to the occlusion site, and this disruption was accompanied by the loss of electrical slow wave. ICC networks and slow waves were normal appearance aboral to the clip. In conclusion, The present results showed that partial intestinal obstruction induced the loss of ICC networks and slow waves. These result will provide a valuable aid for understanding pathogenesis of intestinal motility disorder, and this model may be an important tool for evaluating genetic or molecular factor for the therapeutic opportunities of motility disorder in human.
