Expression of TGF-alpha, p53 and PCNA in Laryngeal Dysplasia.
- Author:
Jong Ouck CHOI
1
;
Kyung Hyun KIM
;
Keun CHUNG
;
Keon CHOI
;
Bom Woo YEOM
;
Heon Ki MIN
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Korea University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
TGF-alpha;
p53;
Proliferating cell nuclear antigen;
Laryngeal dysplasia
- MeSH:
Cell Proliferation;
Epidermal Growth Factor;
G2 Phase;
Larynx;
Lung;
Proliferating Cell Nuclear Antigen*;
Protein-Tyrosine Kinases;
Signal Transduction;
Transforming Growth Factor alpha*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1997;40(6):861-868
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Transforming growth factor(TGF-alpha) is a polypeptide which is structurally related to epidermal growth factor(EGF) and binds to the epidermal growth factor receptor(EGFR). TGF-alpha utilizes EGFR to increase the activation of tyrosine kinase to involve in signal transduction of cellular growth. TGF-alpha synthesis occurs in a variety of neoplastic cells. p53 is a tumor-suppressor gene. There is a strong corrleation between immunohistochemical p53 positivity and p53 mutations in lung and laryngeal carcinoma. PCNA is expressed by cycling cells in late G1, S and G2 phase, and may be used to indicate the degree of cellular proliferation. OBJECTIVES: To evaluate whether TGF-alpha, p53 and PCNA can be used as an indicator to malignant transformation of dysplasia in larynx or not. MATERIALS AND METHODS: The authors investigated the TGF-alpha score, p53 immunoreactivity and PCNA proliferating index by immunohistochemical staining in 30 laryngeal dysplasia from 1992 to 1995. RESULTS: Dysplasia with malignant transformation showed values of 5.46 for TGF-alpha, 29.2 for PCNA proliferating index and 37.5 for p53 immunoreactivity. Dysplasia without malignant transformation showed values of 1.88 for TGF-alpha, 8.6 for PCNA proliferating index and 4.5 for p53 immunoreactivity. Conclusions: The results suggest that TGF-alpha, p53 and PCNA could be an useful indicator to predict the progression of laryngeal dysplasia.
