The effect of kainic acid induced convulsions on the expression of inositol 1,4,5-trisphosphate receptor mRNA in the rat brain.
- Author:
Hyun KIM
1
;
Seung Hun KO
;
Chang Mee KIM
;
Im Joo RHYU
;
Hae Sun CHUNG
;
Dong Yeon KIM
;
Young Suk SUH
Author Information
1. Department of Anatomy, Korea University, College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Kainic acid;
Inositol 1,4,5-trisphosphate receptor;
c-fos;
Dentate gyrus;
in situ hybridization histoche-mistry
- MeSH:
Animals;
Brain*;
Cerebellum;
Cerebral Cortex;
Dentate Gyrus;
Dizocilpine Maleate;
Hippocampus;
In Situ Hybridization;
Inositol 1,4,5-Trisphosphate*;
Inositol*;
Kainic Acid*;
N-Methylaspartate;
Rats*;
Repression, Psychology;
RNA, Messenger*;
Seizures*
- From:Korean Journal of Anatomy
1998;31(5):645-654
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this study, the effect of systemic administration of kainic acid (KA) on the expression of inositol 1,4,5-trisphosphate receptor mRNA in the rat brain was investigated with in situ hybridization histochemistry. After the injection of KA in a convulsive dose (10 mg/kg i.p.), inositol 1,4,5-trisphosphate receptor mRNA was reduced significantly in dentate gyrus, cerebral cortex, and caudate-putamen and moderately in CA1-CA3 areas of hippocampus and cerebellum. In dentate gyrus, the expression of inositol 1,4,5-trisphosphate receptor mRNA was significantly decreased at 6 h, lowest level at 9 h, after that the expression was gradually recovered and returned to basal level at 72 h after KA injection. However, in the CA1-CA3 areas of the hippocampus, cerebral cortex, and caudate-putamen, the expression of inositol 1,4,5-trisphosphate receptor mRNA was abruptly decreased at 9 h and almost return to basal level at 24 h after KA injection. The significant repression of inositol 1,4,5-trisphosphate receptor mRNA in cerebellum was only found at 9 h after KA injection. But significant change of inositol 1,4,5-trisphosphate receptor mRNA was not found in the brains of rats treated with NMDA receptor blocker, MK-801, followed by KA injection. These observations suggest that the inositol 1,4,5-trisphosphate receptor is one of the genes whose expression can be altered by KA treatment and the NMDA receptor is related with this alternation.
