Expression of cytokeratins and involucrin in cultured human keratinocytes.
- Author:
In Hwan SONG
1
;
Yeung Ho DOH
;
Joo Young KIM
;
Yung Chang LEE
;
Eon Gi SUNG
Author Information
1. Department of Anatomy School of Medicine Catholic University of Taegu-Hyosung, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
keratinocytes;
cytokeratin;
involucrin;
in Vitro
- MeSH:
Antigens, Differentiation;
Calcium;
Culture Media, Serum-Free;
Humans*;
Keratinocytes*;
Keratins*
- From:Korean Journal of Anatomy
1998;31(5):663-671
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To evaluate the maturation and differentiation state of cultured keratinocytes, the author investigated expression of differentiation markers in cultured keratinocytes. The specimens were divided into three experimental groups, 3rd passage keratinocytes cultured in serum free media (3rd SFM group), 6th passage keratinocytes cultured in serum free media (6th SFM group) and 3rd passage keratinocytes cultured in DMEM (DMEM group). CK14, marker of basal layer, expressed in all groups. The expression was localized and condensed in the SFM groups but spreade in the DMEM group. Most of the cells in both SFM groups were positive but a few cells in DMEM group were also positive. CK10, marker of initiation of differentiation, expressed weakly in DMEM group but there was no expression in both SFM groups. Involucrin, marker of terminal differentiation, expressed weakly in DMEM group but there was no expression in both SFM groups. CK16 and 17, markers of fast turnover of keratinocytes, were not expressed in SFM groups. Weak positive reactions were observed in DMEM group. With these results the authors concluded that the keratinocytes from 3rd passage to 6th passage, cultured in serum free media with calcium less than 0.1 mM, had highly homogeneous basal cell characteristics.
