Distribution of Brain-Derived Neurotrophic Factor-Immunoreactive Neurons in the Rat Brain after Colchicine Treatment.
- Author:
Jeong Ki KIM
1
;
Won Hark PARK
;
Hee Jung CHO
Author Information
1. Department of Anatomy, School of Medicine, Kyungpook National University, Korea.
- Publication Type:Original Article
- Keywords:
Brain-derived neurotrophic factor;
Brain;
Distribution;
Colchicine;
Immunohistochemistry
- MeSH:
Animals;
Anterior Thalamic Nuclei;
Basal Ganglia;
Brain*;
Brain-Derived Neurotrophic Factor;
Cerebral Cortex;
Colchicine*;
Dentate Gyrus;
Hypothalamus;
Immunohistochemistry;
Midline Thalamic Nuclei;
Neurons*;
Paraventricular Hypothalamic Nucleus;
Rats*;
RNA, Messenger
- From:Korean Journal of Anatomy
1999;32(6):801-809
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Widespread brain-derived neurotrophic factor (BDNF) mRNA and protein expression has been detected in the brain. Despite substantial overlap between BDNF mRNA and protein expression, there is general anatomical regions, where there is discordance of these expression. We performed, therefore, immunohistochemistry after colchicine treatment into the ventricle to evaluate the possible presence of BDNF-immunoreactive (IR) in the regions where BDNF mRNA was expressed, but not BDNF-IR. The results obtained were as follows; There was substantial increase in the number of BDNF-IR neurons in the anterior olfactory nucleus, the piriform cortex, the cerebral cortex, the claustrum, the stratum pyramidale of the CA2 and the CA3, the granule cell layer of the dentate gyrus, the basolateral amygdaloid nucleus, the lateral geniculate nucleus, the anteromedial thalamic nucleus, the anterodorsal thalamic nucleus, the paraventricular thalamic nucleus, the paraventricular hypothalamic nucleus and the ventromedial hypothalamus nucleus, compared to the same brain area of non-colchicine treated rat. We detected many new BDNF-IR neurons in the stratum pyramidale of the CA1, A1, A2, A4-A10 cell groups, C1-C3 cell groups, the raphe magnus nucleus, the lateral paragigantocellular nucleus and the spinal vestibular nucleus. The results show that the localization of BDNF-IR neurons after colchicine treatment is consistant with that of BDNF mRNA containing neurons in the brain.
