Polycaprolactone Triol–Citrate Scaffolds Enriched with Human Platelet Releasates Promote Chondrogenic Phenotype and Cartilage Extracellular Matrix Formation.
10.1007/s13770-017-0023-8
- Author:
Hussin A ROTHAN
1
;
Suhaeb A MAHMOD
;
Ivan DJORDJEVIC
;
Mojtaba GOLPICH
;
Rohana YUSOF
;
Simmrat SNIGH
Author Information
1. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. rothan@um.edu.my rohana@um.edu.my
- Publication Type:Original Article
- Keywords:
Cartilage tissue engineering;
PCLT–CA scaffolds;
PRPr cells;
Human chondrocytes;
ECM formation
- MeSH:
Blood Platelets*;
Cartilage Oligomeric Matrix Protein;
Cartilage*;
Chondrocytes;
Collagen;
Elastomers;
Extracellular Matrix*;
Fibronectins;
Glycosaminoglycans;
Humans*;
Laminin;
Methods;
Phenotype*;
Platelet-Rich Plasma;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Tissue Engineering;
Up-Regulation
- From:
Tissue Engineering and Regenerative Medicine
2017;14(2):93-101
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this paper we report the differentiating properties of platelet-rich plasma releasates (PRPr) on human chondrocytes within elastomeric polycaprolactone triol–citrate (PCLT–CA) porous scaffold. Human-derived chondrocyte cellular content of glycosaminoglycans (GAGs) and total collagen were determined after seeding into PCLT–CA scaffold enriched with PRPr cells. Immunostaining and real time PCR was applied to evaluate the expression levels of chondrogenic and extracellular gene markers. Seeding of chondrocytes into PCLT–CA scaffold enriched with PRPr showed significant increase in total collagen and GAGs production compared with chondrocytes grown within control scaffold without PRPr cells. The mRNA levels of collagen II and SOX9 increased significantly while the upregulation in Cartilage Oligomeric Matrix Protein (COMP) expression was statistically insignificant. We also report the reduction of the expression levels of collagen I and III in chondrocytes as a consequence of proximity to PRPr cells within the scaffold. Interestingly, the pre-loading of PRPr caused an increase of expression levels of following extracellular matrix (ECM) proteins: fibronectin, laminin and integrin β over the period of 3 days. Overall, our results introduce the PCLT–CA elastomeric scaffold as a new system for cartilage tissue engineering. The method of PRPr cells loading prior to chondrocyte culture could be considered as a potential environment for cartilage tissue engineering as the differentiation and ECM formation is enhanced significantly.
