Effects of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system-Based Deletion of miR-451 in Mouse Embryonic Stem Cells on Their Self-Renewal and Hematopoietic Differentiation.
10.1007/s13770-017-0031-8
- Author:
Su Jin KIM
1
;
Chang Hoon KIM
;
Borim AN
;
Kwon Soo HA
;
Seok Ho HONG
;
Kye Seong KIM
Author Information
1. Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, South Korea. ks66kim@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
CRISPR/Cas9;
Pluripotent stem cells;
MicroRNA;
Hematopoiesis
- MeSH:
Animals;
Cell Cycle;
Clustered Regularly Interspaced Short Palindromic Repeats*;
Erythrocytes;
Gene Expression;
Hematopoiesis;
Homologous Recombination;
Humans;
Mice*;
MicroRNAs;
Mouse Embryonic Stem Cells*;
Pluripotent Stem Cells;
Tissue Donors
- From:
Tissue Engineering and Regenerative Medicine
2017;14(2):179-185
- CountryRepublic of Korea
- Language:English
-
Abstract:
Pluripotent stem cells (PSCs) are a useful source of cells for exploring the role of genes related with early developmental processes and specific diseases due to their ability to differentiate into all somatic cell types. Recently, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system has proven to be a robust tool for targeted genetic modification. Here, we generated miR-451-deficient PSCs using the CRISPR/Cas9 system with PCR-based homologous recombination donor and investigated the impact of its deletion on self-renewal and hematopoietic development. CRISPR/Cas9-mediated miR-451 knockout did not alter the gene expressions of pluripotency, cellular morphology, and cell cycle, but led to impaired erythrocyte development. These findings propose that a combination of PSCs and CRISPR/Cas9 system could be useful to promote biomedical applications of PSCs by elucidating the function and manipulating of specific miRNAs during lineage specification and commitment.
