Changes in Lymphocyte DNA Damage and Antioxidant Status after Supplementing Propolis to Korean Smokers: A Placebo-Controlled, Double-Blind Cross-Over Trial.
10.4163/kjn.2009.42.5.442
- Author:
Myung Hee KANG
1
;
Hye Jin LEE
;
Mi Kyung KIM
;
Mi Kyung SUNG
;
Oran KWON
;
Yoo Kyoung PARK
Author Information
1. Department of Food and Nutrition, Hannam University, Daejeon 305-811, Korea.
- Publication Type:Original Article
- Keywords:
propolis;
DNA damage;
smokers;
cross-over;
total antioxidant status (TAS);
catalase;
glutathione peroxidase (GSH-Px);
superoxide dismutase (SOD)
- MeSH:
Ascorbic Acid;
Bees;
Catalase;
Coniferophyta;
Cross-Over Studies;
Dietary Supplements;
DNA;
DNA Damage;
Erythrocytes;
Glutathione Peroxidase;
Lymphocytes;
Oxidative Stress;
Plasma;
Propolis;
Smoke;
Smoking;
Superoxide Dismutase;
Tocopherols;
Trees;
Vitamins
- From:The Korean Journal of Nutrition
2009;42(5):442-452
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Smoking has been known to exacerbate the initiation and propagation of oxidative stresses. Efforts have been made to reduce the smoking-induced oxidative stresses using commercial dietary supplements. Propolis is the resinous substance collected by bees from the leaf buds and bark of trees, especially poplar and conifer trees. In this trial, we examined whether a daily supplementation of 800 mg propolis can protect endogenous lymphocytic DNA damage and modulate antioxidative enzyme activities and the level of antioxidant vitamin in smokers using a placebo-controlled, double-blinded cross-over trial. After two weeks of running-in period, 29 smokers (mean age 34.38 +/- 1.73) received 6 tablets/day of either propolis or placebo pills for 4 weeks. After 2 weeks of washout period the subjects switched they pills for cross-over study. The degree of DNA damage (assessed by tail DNA, tail length and tail moment) was not significantly changed with propolis intake or placebo intake. Similarly, total antioxidant status (TAS) remained at the same level regardless of the treatment. Erythrocyte catalase, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), plasma vitamin C and tocopherol level did not differ before and after propolis treatment, and did not differ between treatments. Putting all these results together, we would suggest that it is still too early to claim that propolis possess antioxidative activities.
