Porous Microcarrier-Enabled Three-Dimensional Culture of Chondrocytes for Cartilage Engineering: A Feasibility Study.
10.1007/s13770-016-0038-6
- Author:
Guang Zhen JIN
1
;
Hae Won KIM
Author Information
1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Korea. kimhw@dku.edu
- Publication Type:Original Article
- Keywords:
Porous microspheres;
Blend biopolymer;
Chondrocyte;
Cartilage engineering
- MeSH:
Aggrecans;
Biopolymers;
Cartilage*;
Cell Culture Techniques;
Chondrocytes*;
Collagen Type II;
Feasibility Studies*;
Gene Expression;
Microspheres
- From:
Tissue Engineering and Regenerative Medicine
2016;13(3):235-241
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cartilage repair is substantially intractable due to poor self-healing ability. Porous microspheres can be a fascinating three-dimensional matrix for cell culture and injectable carrier in cartilage engineering. In this study, we assessed the feasible use of porous biopolymer microspheres for chondrocyte carriers. When seeded onto the blended biopolymer microspheres and followed by a dynamic spinner flask culture, the chondrocytes showed robust growth behaviors during the culture period. The gene expressions of SOX9, type II collagen, and aggrecan were significantly upregulated after 2-week of culture. Furthermore, immunolocalization of type II collagen and secretion of glycosaminolglycan became prominent. The results suggest the feasible usefulness of the porous microspheres as the cell culture matrix and the subsequent delivery into cartilage defects.
