Effects of Estrogen on Intracellular Calcium-Related T-Lymphocyte Function.
10.1007/s13770-015-9070-1
- Author:
Yoon Young KIM
1
;
Hoon KIM
;
Seung Yup KU
;
Chang Suk SUH
;
Seok Hyun KIM
;
Young Min CHOI
;
Sung Joon KIM
Author Information
1. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. jyhsyk@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Estrogen;
T-lymphocyte;
Calcium channel
- MeSH:
B-Lymphocytes;
Calcium;
Calcium Channels;
Cytokines;
Estradiol;
Estrogens*;
Fura-2;
Gonadal Steroid Hormones;
Humans;
Regenerative Medicine;
T-Lymphocytes*;
Tissue Engineering
- From:
Tissue Engineering and Regenerative Medicine
2016;13(3):270-273
- CountryRepublic of Korea
- Language:English
-
Abstract:
Regulation of immune cell function is an important in the field of hormone-related tissue engineering and regenerative medicine. In this sense, hormonal regulation of immune cell function is a critical issue to be solved. It has been known that ovarian sex hormone play an important roles in immune function, however, little has been known whether estrogen affects T-lymphocyte function. Human Jurkat T cells were treated with estradiol (E₂) at concentrations of 0, 10, 100, 1000 ng/mL, and calcium response was evaluated. Intracellular calcium concentrations after Fura-2 acetoxymethyl ester treatment show an increasing trend at higher E₂ concentrations although these alterations did not reach a statistical significance. The expression of calcium channel-related gene CACNA1C did not show any significant changes according to the concentration of E₂. Taken together, estrogen has an implication as a possible hormonal regulator of intracellular calcium release in human Jurkat T cells via non-genomic pathway. Further studies are necessary to investigate the combined effects of sex hormones and cytokines in both T- and B-lymphocytes.
