Local Injection of Granulocyte-Colony Stimulating Factor Accelerates Wound Healing in a Rat Excisional Wound Model.
10.1007/s13770-016-9054-9
- Author:
Guang Yin SHEN
1
;
In Hwa PARK
;
Yi Sun SONG
;
Hyun Woo JOO
;
Yonggu LEE
;
Jeong Hun SHIN
;
Kyung Soo KIM
;
Hyuck KIM
Author Information
1. Department of Cardiology, Hanyang University Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Granulocyte-colony stimulating factor;
Wound healing;
Local injection
- MeSH:
Animals;
Blotting, Western;
Collagen;
Gene Expression;
Granulocyte Colony-Stimulating Factor;
Models, Animal;
Rats*;
Receptors, Granulocyte Colony-Stimulating Factor;
Skin;
Wound Healing*;
Wounds and Injuries*
- From:
Tissue Engineering and Regenerative Medicine
2016;13(3):297-303
- CountryRepublic of Korea
- Language:English
-
Abstract:
A systemic treatment of granulocyte-colony stimulating factor (G-CSF) is known to improve healings of damaged tissues. However, recent studies suggested local actions of G-CSF on the healing processes of damaged tissues. We investigated the treatment effect of locally injected G-CSF and compared to that of systemically injected G-CSF in a rat model. A wound was created on the rat dorsum and treated either by local injection or by systemic injection of G-CSF. Wound healing rate, deposition of collagen, and gene expression were evaluated. G-CSF receptor (G-CSFR) protein was detected by Western blotting. The wound healing rate in the local injection group was significantly higher than that in the systemic injection group at days 9 and 15; it was also significantly higher than that in the control group at days 3, 9, and 15. The expression of G-CSFR protein in wound tissues was higher than in normal skin tissues. The local injection of G-CSF is more effective than systemic injection of G-CSF in promoting wound healing, which may implicate the local action of G-CSF treatment in wound healing processes.
