Neural Differentiation of Mesenchymal Stem Cells from Bone Marrow of Human Mastoid Process.
- Author:
Hyong Ho CHO
1
;
Han Seong JEONG
;
Su Jeong JANG
;
Jong Seong PARK
;
Hyu Chae CHO
;
Chul Ho JANG
;
Yong Bum CHO
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Gwangju, Korea. choyb@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Cell differentiation;
Temporal bone;
Mastoid
- MeSH:
Bone Marrow;
Cell Differentiation;
Epidermal Growth Factor;
Fibroblast Growth Factors;
Forskolin;
Glial Fibrillary Acidic Protein;
Humans;
Immunohistochemistry;
Mastoid;
Mesenchymal Stromal Cells;
Nervous System Diseases;
Neurofilament Proteins;
Neurons;
Otitis Media;
Regenerative Medicine;
RNA, Messenger;
Sodium;
Temporal Bone
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2008;51(5):422-428
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Reports of neural differentiation of mesenchymal stem cells suggest the possibility that these cells may serve as a source for stem cell-based regenerative medicine to treat neurological disorders. The purpose of this study was to generate neural cells by differentiation of bone marrow-derived mesenchymal stem cells that isolated from human mastoid process. MATERIALS AND METHOD: Human mesenchymal stem cells (hMSCs) isolated from human mastoid process bone marrow during mastoidectomy for chronic otitis media surgery were characterized using fluorescence-activated cell sorter. Induction of neural differentiation from hMSCs was performed using mitogenic factors (basic fibroblast growth factor, epidermal growth factor, forskolin, isobutylmethylxanthine), and the characterization of differentiated hMSCs was performed using immunohistochemistry, RT-PCR and whole cell patch clamp technique. RESULTS: hMSCs from bone marrow of mastoid process were isolated and cultured. Differentiated cells from hMSCs expressed mRNA transcripts for neuron specific markers, TUJ1 and neurofilament proteins (NF-L, NF-M) as determined by RT-PCR, and neuron specific markers, suhc as NeuN, TUJ1, microtubule-associated protein-2 (MAP2) and glial fibrillary acidic protein by immunohistochemistry. These cells showed voltagedependent sodium currents that was blocked by tetrodotoxin. CONCLUSION: hMSCs, which were isolated from human mastoid process bone marrow, were one of the good sources for stem cell-based regenerative medicine to treat neurological disorders.
