Expression Patterns of KCNJ10 K+ Channel in the Cochlear Lateral Wall After Acoustic Trauma.
- Author:
Bon Seok KOO
1
;
Ah Young KIM
;
Jae Yong PARK
;
Sang Hee LEE
;
Jin Man KIM
;
Yong Min KIM
;
Ki Sang RHA
;
Yong Ho PARK
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Chungnam National University College of Medicine, Daejeon, Korea. parkyh@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Noise;
Cochlea;
KCNJ10
- MeSH:
Acoustics;
Adult;
Animals;
Cochlea;
Endolymph;
Evoked Potentials, Auditory, Brain Stem;
Guinea Pigs;
Hair;
Hearing Loss, Noise-Induced;
Homeostasis;
Humans;
Male;
Neurons;
Noise;
ortho-Aminobenzoates;
Spiral Ligament of Cochlea;
Stria Vascularis
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2008;51(4):312-318
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: It is well known that noise exposure leads to the sensory hair cell loss and other neuronal damage in the cochlea. But recently it has been reported that noise exposure could also damage lateral wall of cochlea such as stria vascularis and spiral ligament. K+ is the major cation in endolymph and important to maintain homeostasis within the cochlea. We have investigated the expression patterns of KCNJ10 K+ channel in noise induced cochlear damage. MATERIALS AND METHOD: Twenty adult male guinea pigs (300-350 g) were included in this study. In experimental group (n=16), acoustic trauma was induced by continuous broad band noise for 2 hr to 115 dB SPL and broad band noise for 6 hr to 120 dB SPL with 3 consecutive days. After noise exposure, auditory brainstem response threshold shift and hair cell loss were evaluated. A study for KCNJ10 K+ channel expression was examined by immunohistochemical staining. RESULTS: After noise exposure, auditory brainstem response showed transient threshold shift (TTS) and permanent threshold shift (PTS) in accordance with noise exposure. The expression patterns of CKNJ10 K+ channel were changeable in TTS group. But there were no change of expression patterns in PTS group. CONCLUSION: In the cochlear lateral wall, KCNJ10 K+ channel expressions were affected with noise exposure and these changes might be associated with the regulation of homeostasis in the cochlea lateral wall.
