Effects of Dietary Folate Supplementation on the Homocystine Diet-Induced Hyperhomocysteinemia and Hepatic S-Adenosylmethionine Metabolism in Rats.
- Author:
Ji Myung KIM
1
;
Hwa Young LEE
;
Namsoo CHANG
Author Information
1. Department of Food and Nutritional Science, Ewha Women University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
hyperhomocysteinemia;
folate;
S-adenosylmethionine (SAM);
S-adenosylhomocysteine (SAH)
- MeSH:
Animals;
Diet;
Folic Acid*;
Homocysteine;
Homocystine*;
Hyperhomocysteinemia*;
Metabolism*;
Plasma;
Rats*;
S-Adenosylhomocysteine;
S-Adenosylmethionine*;
Vitamin B 12
- From:The Korean Journal of Nutrition
2003;36(8):811-818
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We investigated the effects of dietary folate supplementation on plasma homocysteine, vitamin B12 and hepatic levels of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in diet-induced hyperhomocysteinemic rats. All animals were fed 0.3% homocysteine diet for 2 weeks, then they were placed either on a 0.3% homocystine or no homocystine with or without 8 mg/kg folate diet for 8 weeks. Homocystine diet induced hyperhomocysteinemia up to 3.5-fold at 10 weeks (28.0+/-4.8 micromol/l vs. 7.9+/-0.3 micromol/l). Dietary folate supplementation caused a significant decrease in plasma homocysteine levels which had been increased by a homocystine-diet. Also, dietary folate supplementation made them return to control levels at 4 wk when the diet was free of homocystine. Plasma folate levels were markedly decreased with homocystine diet with no folate supplementation. Plasma vitamin B12 did not differ between groups. Dietary homocystine increased hepatic levels of SAM in folate supplementation group at 10 weeks (p<0.05). Dietary folate supplementation increased hepatic levels of SAM/SAH ratios in homocystine group (p<0.05). In conclusion, dietary folate supplementation can effectively ameliorate the detrimental effects of hyperhomocysteinemia.mia.
