Study on the Role of Estrogen Receptor-Alpha in Yak-Kong and Soybean Induced Proliferation of MG-63 Human Osteoblastic Cells.
- Author:
So Jung UM
1
;
In Sook KANG
;
Yunhi CHO
Author Information
1. Department of Medical Nutrition, Graduate School of East-West Medical Science, Seoul, Korea. choyunhi@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Yak-kong;
soybean;
isoflavone;
ERalpha;
MG-63 cell proliferation
- MeSH:
Cell Line;
Cell Proliferation;
Estrogen Receptor alpha;
Estrogen Receptor beta;
Estrogens*;
Female;
Genistein;
Humans*;
Insulin-Like Growth Factor I;
Isoflavones;
Medicine, Traditional;
Osteoblasts*;
Osteoporosis, Postmenopausal;
Phytoestrogens;
RNA;
Soybeans*
- From:The Korean Journal of Nutrition
2005;38(7):512-520
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Phytoestrogens, especially Yak-kong or soybean-derived isoflavones have been traditionally used as a supplement of estrogen for preventing postmenopausal osteoporosis in oriental folk medicine. In a previous study, we demonstrated that as Yak-kong and soybean increased MG-63 human osteoblastic cell proliferation, the expression of estrogen receptor alpha and beta (ERalpha: ERbeta) both were increased. However, the increased level of ERalpha is much higher than that of ERbeta. To determine whether the altered level of ERalpha expression affects Yak-kong or soybean induced MG-63 cell proliferation, we established cell lines stably expressing either ERalpha or antisense ERalpha RNAs. Increased expression of ERalpha in MG-63 cells (ERalpha-MG63) enhanced Yak-kong or soybean induced proliferation which paralleled with the enhanced expression of IGF-I. Inhibition of ERalpha expression by antisense ERalpha RNAs (As-ERalpha-MG63) caused these cells to insensitize Yakkong or soybean induced proliferation and IGF-I expression. Furthermore, the comparable effects between Yak-kong and the combined treatment of genistein and daidzein at 0.5 x 10-8M, which is a concentration of these two isoflavones similar to Yak-kong at 0.001 mg/ml, on cell proliferation and IGF-I expression in ERalpha-MG63 or As-ERalpha-MG63 cells demonstrate that ERalpha plays an important, active role in MG-63 cell proliferation induced by phytoestrogens, especially Yak-kong or soybean derived isoflavones.
