Characteristic Features of Immune B Cells in Murine Cervical Lymph Node.
- Author:
Jong Bin KIM
1
;
Seung Keun YEO
;
Sung Wan KIM
;
Joong Saeng CHO
;
Chang Il CHA
Author Information
1. Department of Otolaryngology, College of Medicine, Kyung Hee University, Seoul, Korea. khuent@khmc.or.kr
- Publication Type:Original Article
- Keywords:
B cell;
Murine;
Cervical lymph node
- MeSH:
Animal Experimentation;
Animals;
B-Lymphocytes*;
Cell Proliferation;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Immunoglobulin A;
Immunoglobulin G;
Immunoglobulin M;
Immunoglobulins;
Lymph Nodes*;
Mice;
S Phase;
Thymidine
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2005;48(2):241-246
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: To investigate the characteristic features of B cells in murine cervical lymph node. Animal experiment. SUBJECTS AND METHOD: We obtained cervical lymph node of mice. Isolated B cells were cultured in media after the addition of several stimuli. The expression of various surface molecules to characterize lymphoid B cells were analysed by flow cytometry. Immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. B220+ cells cultured for 5 days with media alone, or LPS. Entering into S phase in response to stimuli was measured by proliferative assay. RESULTS: Identifying phenotypic characteristics for B cells of lymph node include CD5low, CD23high, CD43low, B220high, Ig (sIg)Mlow, sIgDhigh, CD80 (B7.1)low, CD86 (B7.2)low, and Syndecam-1low. Spontaneous Immunoglobulin production did not occur in lymphoid B cells. When stimulation, IgM secretion was increased more than that of IgA and IgG. Lipopolysaccharide is a most powerful stimulus which secreted immunoglobulin. In thymidine incorporation, B cells were entered actively S phase after 48hours stimulation. CD40 plus anti-CD8 stimulated B cells to produce substantial thymidine incorporation that was much greater than those of other stimuli. CONCLUSION: The B cells in cervical lymph node are conventional B cells (B2). Lymph node B cells secreted mainly IgM at stimuli and cell proliferation was activated at 48 hours of stimulation.
