The Suppressive Effect of Alendronate Sodium on Nitric Oxide Release by Macrophage.
10.4055/jkoa.2004.39.2.222
- Author:
Ick Hwan YANG
1
;
Soo Hyun LEE
;
Chang Dong HAN
Author Information
1. Department of Orthopaedic Surgery, Yonsei University, College of Medicine, Seoul, Korea. ihyang@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Osteolysis;
Nitric oxide;
Macrophage;
Alendronate Sodium
- MeSH:
Administration, Oral;
Alendronate*;
Animals;
Arthroplasty;
Cell Survival;
Centrifugation;
Centrifugation, Isopycnic;
Dogs;
Macrophages*;
Monocytes;
Nitric Oxide*;
Osteolysis;
Titanium
- From:The Journal of the Korean Orthopaedic Association
2004;39(2):222-227
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was designed to present the effect of alendronate sodium on prevention of osteolysis after arthroplasty by suppression of macrophage. MATERIALS AND METHODS: Submicron-sized titanium particles were made from TiAlV cylinder and plate after milling, and by repeated washing, centrifugation and floating method. 12 dogs were given alendronate by oral administration for 3 months and blood was sampled at 3 weeks, 6 weeks and 3 months. Monocytes were separated from canine blood by isopycnic centrifugation with Percoll solution and cultured in media with LPS, L-NMA, TiAlV particle (x1 SAR and x5 SAR) for 24 hours. Nitric Oxide (NO) assay by Mendelow's method and MTT assay as a cell viability test were performed. RESULTS: Average diameter of TiAlV particles was 1.1+/-0.8 m (from 0.17 to 4.0 m). Particles less than 1.0 m were 55% and 3.0 m, 96%. Compared with control group, there was slight decrease of NO production in 3 weeks group with no statistic difference, but there was significant decrease in 6 weeks and 3 months group statistically. CONCLUSION: Administration of alendronate for more than 6 weeks may be effective in suppression of macrophage, prevention of osteolysis after arthroplasty.
