The Relationships between Homocysteine, Folate, MTHER and TSER Polymorphism for Osteoporotic Compression Fracture in Postmenopausal Women.
10.4055/jkoa.2007.42.5.665
- Author:
Dong Eun SHIN
1
;
Duck Yun CHO
;
Hyung Ku YOON
;
Nam Gun KIM
;
In Seok LEE
Author Information
1. Department of Orthopedic Surgery, Bundang CHA Hospital, College of Medicine, Pochon CHA University, Seongnam, Korea. shinde@cha.ac.kr
- Publication Type:Original Article
- Keywords:
Osteoporotic fracture;
Homocysteine;
Folate;
MTHFR;
TSER
- MeSH:
Female;
Folic Acid*;
Fractures, Compression*;
Genotype;
Homocysteine*;
Humans;
Osteoporotic Fractures;
Plasma;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length;
Sepharose
- From:The Journal of the Korean Orthopaedic Association
2007;42(5):665-670
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To analyze the relationships between homocysteine, folate, MTHFR and TSER polymorphism for postmenopausal women with osteoporotic compression fractures. MATERIALS AND METHODS: Forty-three postmenopausal compression fracture patients and as many normal controls were included. The plasma homocysteine and folate levels were measured using a FPIA (fluorescent polarizing immunoassay) kit. The MTHFR and TSER genotypes were amplified by PCR (polymerase chain reaction) and separated by RFLP (restriction fragment length polymorphism) in a 3.5% agarose gel. RESULTS: The plasma folate level was significantly lower in the postmenopausal women with osteoporotic compression fractures, particularly in the MTHFR 677CT and TSER 2R (-) genotypes. However, the plasma homocysteine level and MTHFR C677T polymorphism were similar to the control group. CONCLUSION: A low folate level and the TSER 2R (-) genotype can be associated with osteoporotic compression fractures in postmenopausal women.
