Inhibition of Allergic Response by CpG Motif Immunostimulatory Oligodeoxynucleotide in a Murine Model of Allergic Rhinitis.
- Author:
Ja Bock YUN
1
;
Jeong Whun KIM
Author Information
1. Department of Otorhinolaryngology, Inje University, Sanggye Paik Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Dermatophagoides farinae;
Allergic rhinitis;
Murine model;
CpG motif;
Immunostimulatory sequence
- MeSH:
Anaphylaxis;
Animals;
Base Sequence;
Cell Proliferation;
Dermatophagoides farinae;
DNA;
Eosinophils;
Hypersensitivity;
Immunoglobulin E;
Immunotherapy;
Korea;
Mice;
Nasal Mucosa;
Pruritus;
Pyroglyphidae;
Rhinitis*;
Vaccination
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2002;45(5):462-466
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The classic immunotherapy has the inconvenience and the risk of anaphylaxis. Recent reports revealed that immunostimulatory DNA sequences (CpG motif, ISS-ODN) have been shown to act as strong Th1 response-inducing adjuvants and DNA based vaccination might be an effective therapeutic option for the treatment of allergic disease. In our study, we investigated whether ISS-ODN have anti-allergic effect in the mouse rhinitis model of Dermatophagoides farinae (Der f), the most common allergen in Korea. MATERIALS AND METHOD: C57BL/6 mice were sensitized with crude extract of Der f extract. After injection of ISS-ODN, several parameters of allergic response were evaluated. RESULTS: Injection with ISS-ODN suppressed itching symptom, eosinophilic infiltration into nasal mucosa and the splenic T cell proliferation after Der f allergen challenge in the murine model of allergic rhinitis. However, injection with ISS-ODN did not show a detectable Der f-specific IgE suppression. CONCLUSION: Our findings suggested ISS-ODN have an anti-allergic effect in the allergic rhinitis model of Der f allergen. House dust mite allergy can be reduced by the injection with ISS-ODN.
