In vitro cytotoxicity of self-etching primers.
- Author:
Chang Hoon RHEE
1
;
In Ryeon KIM
;
Gyoo Cheon KIM
;
Seong Sik KIM
;
Woo Sung SON
Author Information
1. Department of Orthodontics, School of Dentistry, Pusan National University, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Cytotoxicity;
Self-etching primer
- MeSH:
Atrophy;
Ions;
Mouth;
Necrosis
- From:Korean Journal of Orthodontics
2006;36(6):422-433
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Several ions and components are released from self-etching primers in the oral cavity. This may cause injury to the periodontal tissues throughout orthodontic treatment. The purpose of this study was to assess the cytotoxicity of self-etching primers to HGF-1, HaCaT, and RHEK cells. METHOD: Transbond XT Primer (3M Unitek, Monrovia, CA, USA), and self-etching primers, Clearfil SE Bond (Kuraray, Osaka, Japan), Transbond Plus SEP (3M Unitek, Monrovia, CA, USA), and Adper Prompt L-Pop (3M Unitek, Monrovia, CA, USA), were evaluated by MTT assay, and cellular changes were also observed. RESULTS: In all cells after 72 hours with all primers, severe morphological changes such as atrophy and necrosis were observed. In the MTT assay using HGF-1, Clearfil SE Bond, Transbond XT Primer, Transbond Plus SEP, and Adper Prompt L-Pop were lined up in order of ascending cytotoxicity.When using HaCaT, Clearfil SE Bond, Adper Prompt L-Pop, Transbond Plus SEP, and Transbond XT Primer were lined up in order of ascending cytotoxicity. When using RHEK, Clearfil SE Bond, Transbond XT Primer, Adper Prompt L-Pop, and Transbond Plus SEP were lined up in order of ascending cytotoxicity. CONCLUSION: The result of this study shows that care is needed because self-etching primers show cytotoxic properties similar to conventional primers.
