Effects of nitric oxide on the proliferation and differentiation of human periodontal ligament cells.
- Author:
Sun Young CHOI
1
;
Jin Hyoung CHO
;
Kyung Hwa KANG
Author Information
1. Department of Orthodontics, School of Dentistry, Wonkwang University, Korea. occlplane@hanmail.net
- Publication Type:Original Article
- Keywords:
Nitric oxide;
Human periodontal ligament cells;
Growth and differentiation
- MeSH:
Alkaline Phosphatase;
Cell Survival;
Humans*;
Integrin-Binding Sialoprotein;
Nitric Oxide*;
Nitroprusside;
Osteonectin;
Periodontal Ligament*;
Tissue Donors;
Tooth Movement
- From:Korean Journal of Orthodontics
2006;36(6):465-473
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study evaluated the effects of nitric oxide (NO) on the proliferation and differentiation of human periodontal ligament cells involved in orthodontic tooth movement. METHODS: A range of concentrations of sodium nitroprusside (SNP), a NO donor, were administered to samples of human periodontal ligament cells, followed by measurement of cell viability, alkaline phosphatase (ALP) activity, and expression of osteonectin and bone sialoprotein. RESULTS: Cell viability, ALP activity and the expression of osteonectin and bone sialoprotein were increased in human periodontal ligament cells treated with SNP concentrations of < 0.2 mM compared with controls, but were decreased with SNP concentrations of > 1.0 mM. CONCLUSION: NO has a biphasic effect on proliferation and differentiation in human periodontal ligament cells, with a stimulatory effect at low concentrations and an inhibitory effect at high concentrations.
