Induction of Mucosal Tolerance Using House Dust Mite-Cholera Toxin B Conjugate in Mouse Model of Allergic Rhinitis: Prophylactic and Long-Term Therapeutic Effects.
- Author:
Chul Hee LEE
1
;
Seung Jun OH
;
Jae Goo KANG
;
Jong Lyel ROH
;
Seung Sin LEE
;
Song Hwa QUAN
;
Chae Seo RHEE
;
Yang Gi MIN
;
Seung Yong SUNG
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea. chulhee@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Dermatophagoides farinae;
Allergy;
Cholera toxin;
Immunotherapy
- MeSH:
Administration, Mucosal;
Animals;
Cell Proliferation;
Cholera Toxin;
Dermatophagoides farinae;
Dust*;
Eosinophils;
Hypersensitivity;
Immunoglobulin E;
Immunotherapy;
Mice*;
Nasal Mucosa;
Pyroglyphidae;
Rhinitis*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2004;47(3):232-237
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Cholera toxin B (CTB) is an effective immunomodulating agent. Mucosal tolerance is a well recognized method for inducing tolerance, but large amounts of antigen is needed. The dosage required can be dramatically reduced through coupling the antigen to the CTB. This study aimed to examine whether mucosal administration of house dust mite coupled to CTB (HDM-CTB conjugate) would modulate specific type 1 hypersensitivity in the murine model of allergic rhinitis and to evaluate the prophylactic and long-term therapeutic effects. MATERIALS AND METHOD: C57BL/6 mice were sensitized with Dermatophagoides farinae extract. After administration of HDM-CTB conjugate, several parameters of allergic response were evaluated. RESULTS: After mucosal adminstration of HDM-CTB conjugate, allergic symptoms, eosinophilic infiltration into nasal mucosa, specific IgE, and the splenic T cell proliferation after Dermatophagoides farinae allergen challenge were suppressed. CONCLUSION: These findings show that HDM-CTB conjugate has an anti-allergic effect in the murine model of allergic rhinitis and suggest that house dust mite allergy can be modulated with mucosal administration of allergen coupled to CTB.
