Coexistence of Tyrosine Hydroxylase and Nicotinamide Adenine Dinucleotide Phosphate-Diaphorase in Hypothalamic Neurons of the Rat.
- Author:
Young Buhm HUH
1
;
Jung Sik CHO
;
Chang Sub SONG
;
Hee Kyung AHN
Author Information
1. Department of Anatomy, College of Medicine, Kyung-Hee University, Kohwang Medical Research Institute, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
NADPH-diaphorase;
Tyrosine hydroxylase;
Coexistence;
Hypothalamus
- MeSH:
Animals;
Anterior Hypothalamic Nucleus;
Dorsomedial Hypothalamic Nucleus;
Hypothalamus;
Immunohistochemistry;
NAD*;
Neurons*;
Niacinamide*;
Nitric Oxide;
Nitric Oxide Synthase;
Paraventricular Hypothalamic Nucleus;
Preoptic Area;
Rats*;
Supraoptic Nucleus;
Tyrosine 3-Monooxygenase*;
Tyrosine*
- From:Korean Journal of Anatomy
1998;31(1):119-126
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The presence and coexistence of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-diaphorase) with tyrosine hydroxylase (TH) was investigated by combining NADPH-diaphorase histochemistry with TH immunohistochemistry in hypothalamic nuclei of the rat. TH-immunoreactive and NADPH-diaphorase positive neurons were found in the medial preoptic area and medial preoptic nucleus, anterior hypothalamic area, dorsomedial hypothalamic nucleus, paraventricular nucleus, supraoptic nucleus and posterior hypothalamic area, respectively. TH and NADPH-diaphorase did not coexist in the anterior hypothalamic area, dorsomedial hypothalamic nucleus, medial preoptic area and posterior hypothalamic area. A considerable portion (30~50%) of the NADPH-diaphorase positive neurons in the supraoptic nucleus colocalized TH. In the medial preoptic area and paraventricular nucleus, some (5~15%) of TH-immunoreactive neurons also contained NADPH-diaphorase activity. NADPH-diaphorase is known to be an indicator of the enzyme nitric oxide synthase; these results therefore suggest that nitric oxide may play an important role in the regulation of the activity of the hypothalamic dopaminergic system of the rat.
