The Difference between the Concentrations of Arachidonic Acid Metabolites in the Cholesterol Granuloma and Granulation Tissues in Chronic Otitis Media.
- Author:
Yong Ju JANG
1
;
Han Gyun KIM
;
Jang Keun OH
;
Chung Ku RHEE
Author Information
1. Department of Otolaryngology, Dankook University School of Medicine, Cheonan, Korea. yjang@anseo.dankook.ac.kr
- Publication Type:Original Article
- Keywords:
Arachidonic acids;
cholesterol granuloma;
granulation tissue
- MeSH:
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid;
Arachidonic Acid*;
Arachidonic Acids;
Bone Resorption;
Cholesterol*;
Chromatography, Liquid;
Cytochrome P-450 CYP1A1;
Dinoprostone;
Granulation Tissue*;
Granuloma*;
Humans;
Leukotriene D4;
Leukotriene E4;
Lipoxygenase;
Mastoid;
Metabolism;
Otitis Media*;
Otitis*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2001;44(5):466-469
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Both the granulation tissue and cholesterol granuloma can erode the surrounding bone and ossicles. However, the etiology of bone resorption in the granulation tissue and cholesterol granuloma has not been evident. The aim of this study was to assay the concentrations of arachidonic acid metabolites (AAMs) in cholesterol granuloma and the mastoid granulation tissue in order to better understand the possible role of AAMs in the pathophysiology of cholesterol granuloma in comparison with the granulation tissue. MATERIALS AND METHOD: Cholesterol granuloma tissues were obtained from eight patients who had underwent tympanomastoidectomy. Granulation tissues, which served for comparison, were taken from 12 patients who had underwent tympanomastoidectomy. Tissue concentrations of prostagladin (PG)E2, 6-keto-PGF1alpha, leukotriene (LT)C4, LTD4, LTE4, 15-hydro xyeicosatetraenoic acid (HETE), 12-HETE, 5-HETE, and thromboxane (TXB)2 were calculated using high performance liquid chromatography and compared between cholesterol granuloma and granulation tissue. RESULT: The level of 12-HETE was higher in cholesterol granuloma than in the granulation tissue. Among the PGs, the tissue concentration of PGE2 was particularly high in cholesterol granuloma than in the granulation tissue. LTD4 was the only LT detectable in cholesterol granuloma. In comparison to the AAMs in cholesterol granuloma, the lipoxygenase pathway products such as 12-HETE, 15-HETE, and 5-HETE were present in lower concentrations in the granulation tissue. LT was undetectable in the granulation tissues. CONCLUSION: Our results suggest that cholesterol granuloma and the granulation tissue is different not only in terms of histology, but also in terms of biochemical properties such as arachidonic acid metabolism.
