Interleukin-13 Suppresses MUC5AC Gene Expression and Mucin Secretion in Cultured Normal Human Nasal Epithelial Cells.
- Author:
Jeung Gweon LEE
1
;
Chang Hoon KIM
;
Hyun Ung KIM
;
Jae Young CHOI
;
Jungho BAE
;
Joo Heon YOON
Author Information
1. Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Interleukin-13;
Mucins;
MUC5AC
- MeSH:
Asthma;
Epithelial Cells*;
Gene Expression*;
Humans*;
Inflammation;
Interleukin-13*;
Models, Animal;
Mucins*;
Mucus;
Rhinitis;
RNA, Messenger
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2001;44(11):1150-1156
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: It is not clear which mucins are responsible for the mucus hypersecretion in allergic rhinitis and allergic asthma. Recently, it was found that IL-13 is closely related to allergic inflammation and mucus hypersecretion in in vivo animal models. However, the role of IL-13 in in vitro cellular models has yet to be determined. This study aimed to investigate the direct effect of IL-13 on mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells. MATERIALS AND METHOD: Cells were cultured with passage-2 normal human nasal epithelial cells using the air-liquid interface culture method. After treatment with IL-13, the total mucin and MUC5AC mucin levels were measured using the immuno-blotting assay. The MUC2, MUC5AC, and MUC8 mRNA expressions were measured using RT-PCR. Immunostaining was also performed using a MUC5AC antibody on histologic and cytospin slides. RESULTS: After treatment with 5 ng/ml or more of IL-13, the level of total mucin and MUC5AC mucin secretion decreased substantially. The expression of MUC2 and MUC8 mRNA increased with higher concentrations of IL-13, but the expression of MUC5AC mRNA decreased. On the 7th day after IL-13 treatment, a significant decrease in the number of MUC5AC-positive cells was confirmed with immunostaining. CONCLUSION: These findings indicate that IL-13 suppresses MUC5AC mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells in vitro.
