Localization of Cyclooxygenase Isozymes in Skin Wound Healing in Mouse.
- Author:
Jun Hyuk KOH
1
;
Bek Hyun CHO
;
Tag HEO
;
Mi Ok BAE
;
Song Eun LEE
;
Kyu Youn AHN
;
Choon Sang BAE
;
Baik Yoon KIM
;
Sung Sik PARK
;
Kwang Il NAM
Author Information
1. Department of Anatomy, Chonnam National University Medical School. atlas@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Cyclooxygenase;
Wound healing;
Keratinocyte differentiation;
Western blot;
Immunohistochemistry
- MeSH:
Animals;
Blotting, Western;
Endothelial Cells;
Epidermis;
Fibroblasts;
Immunohistochemistry;
Isoenzymes*;
Keratinocytes;
Mice*;
Prostaglandin-Endoperoxide Synthases*;
Prostaglandins;
Skin*;
Wound Healing*;
Wounds and Injuries*
- From:Korean Journal of Anatomy
2003;36(2):115-122
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cyclooxygenase (COX)-1 and -2 expressions in the incisional wound healing of mouse skin were determined by immunohistochemistry and Western blot analysis. By Western blotting, compared to normal skin, COX-2 activity was increased at days 1, 4, 8, and 12 and was maximal at 4 day after incisional wound of mouse skin whereas COX-1 was barely detectable. In normal skin, COX-1 immunostaining was observed among the basal cells of epidermis whereas COX-2 immunostaining was detected in the more differentiated, suprabasal keratinocytes. At 1~4 days after wound, COX-2 staining was particularly prominent in the inflammatory cells, and at day 8, many macrophage-like cells were stained positively. COX-2 immunoreactive fibroblast, macrophage-like cells, and newly formed vascular endothelial cells were increased in number at 12 days after incision. These data suggest that COX-2 is constitutively expressed, just as is COX-1, in epidermis and is associated with keratinocyte differentiation. In addition, these findings support the well-established role for COX-2, the prostaglandins that they generate, as mediators of inflammatory response.
