Regulation of CC Chemokines in TDI-Induced Nasal Hyperreactive Rats: Expression of RANTES and Eotaxin mRNA Examined Using Competitive PCR.
- Author:
Chan Seung HWANG
1
;
Hang PARK
;
Seng Ho PARK
;
Jung Hoon LEE
;
Young Ho HONG
;
Hoon KIM
;
Hak Hyun JUNG
;
Sung Joon PARK
Author Information
1. Department of Otolaryngology, College of Medicine, Chung-Ang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
TDI;
Eosinophil;
RANTES;
Eotaxin
- MeSH:
Animals;
Chemokine CCL5*;
Chemokines;
Chemokines, CC*;
Chemotactic Factors;
Eosinophilia;
Eosinophils;
Humans;
Inflammation;
Leukocytes;
Mucous Membrane;
Negotiating;
Polymerase Chain Reaction*;
Rats*;
RNA, Messenger*;
Turbinates
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1999;42(8):985-992
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Chemokines are effective leukocyte chemoattractants and may play an important role in mediating eosinophil recruitment in various allergic conditions in human. Eotaxin is an eosinophil-specific chemokine associated with the recruitment of eosinophils to the site of allergic inflammation. However, it is not yet known as to whether or not RANTES is associated with selective tissue eosinophilia. The aim of this study is to understand the events involved in selective eosinophil migration into inflammatory sites. MATERIALS AND METHODS: We performed the quantitative analysis of RANTES and eotaxin mRNA expression levels in TDI-induced nasal hyper-reactive rats. Expression levels of RANTES and eotaxin mRNA from inferior turbinate mucosa were examined using competitive PCR in 35 experimental rats and 5 control rats compared with infiltrated eosinophil counts. RESULTS: The quantity of RANTES mRNA increased 3 folds 2 day after provocation, and the infiltrating eosinophils were correlated with the expression levels of RANTES mRNA (p<0.01). The quantity of eotaxin mRNA increased 15 folds 1 day after provocation. These results suggest that RANTES and eotaxin play a role in controlling antigen-induced eosinophil recruitment into the tissue. Eotaxin is a more potent and selective chemoattractant for eosinophils than infiltrating eosinophils, and were correlated with the expression levels of eotaxin mRNA (p<0.01). CONCLUSIONS: Further investigations for chemokine receptor related to eosinophils will provide better understanding of the mechanism involved in selective tissue eosinophilia.
