The Control of Tumor Cell Invasiveness in Chondrosarcoma Cell Lines by Modifying Focal Adhesion Kinase Expression.
10.4055/jkoa.2002.37.4.552
- Author:
Yun Hee KIM
1
;
Hee Boong PARK
;
Kyu Ho SHIN
;
Soo Bong HAHN
;
Jin Woo LEE
Author Information
1. Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul, Korea. ljwos@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Chondrosarcoma cell line;
cell attachment;
cell invasion;
focal adhesion kinase;
integrin-beta 1;
FAK-CD
- MeSH:
Adenoviridae;
Adenoviridae Infections;
Antibodies;
Cell Line*;
Chondrocytes;
Chondrosarcoma*;
Collagen Type II;
Focal Adhesion Protein-Tyrosine Kinases*;
Focal Adhesions*;
Peptides;
Phosphorylation;
Transfection;
Tyrosine;
Wound Healing
- From:The Journal of the Korean Orthopaedic Association
2002;37(4):552-558
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We propose that cell attachment and invasion can be regulated by the modulation of FAK expression in chondrosarcoma cell lines. MATERIALS AND METHODS: The C-terminal domain of FAK (FAK-CD) was transfected by recombinant adenovirus infection in chondrosarcoma cell lines, JJ012 and 105KC. The expression of FAK, FAK-CD and tyrosine phosphorylation were checked. Chondrocytes and chondrosarcoma cells were used in cell attachment tests by blocking or not blocking integrin-beta 1 antibodies and synthetic peptides on type II collagen. To evaluate the effect of cell invasiveness, a wound healing assay and a Boyden chamber assay were done after FAKCD transfection. RESULTS: We observed higher FAK expression in the chondrosarcoma cells than in chondrocytes. The level of attachment to type II collagen was significantly inhibited by blocking with the antibody of integrin-beta 1 and synthetic RGD peptides. Also, the adenovirus mediated transfection of FAK-CD resulted in the inhibition of the phosphorylation of FAK and significant inhibition of cell attachment in only JJ012, without changing FAK expression. Moreover, migration after transfection with FAK-CD was reduced by up to 79.9% for JJ012 and 75.5% for 105KC. CONCLUSION: Attachment of chondrosarcoma cells could be mediated through integrin-beta 1. We conclude that modified FAK expression contributes to the suppression of tumor cell attachment and invasion.
