Effect of Pyroligneous Liquor on Oxygen Radicals and Their Scavenger Enzymes in Liver of CD Rats.
- Author:
Weon Ki CHO
1
;
Jin Ho CHOI
Author Information
1. Clinical Dualism Research Institute, Choa Pharmacy Co. Ltd, Seoul 150-992, Korea. byunmi@hanmail.net
- Publication Type:Original Article
- Keywords:
pyroligneous liquor;
superoxide radical;
hydroxyl radical superoxide dismutase (SOD );
glutathione pe-roxidase
- MeSH:
Animals;
Cats;
Cytosol;
Diet;
Free Radicals;
Humans;
Hydroxyl Radical;
Liver*;
Male;
Microsomes;
Mitochondria;
Oxygen*;
Rats*;
Reactive Oxygen Species*;
Superoxide Dismutase;
Superoxides;
Water
- From:The Korean Journal of Nutrition
2007;40(2):111-117
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was designed to investigate the effects of pyroligneous liquor on oxygen radicals and their scavenger enzy-mes in the liver of Cri/Bgi CD rats (7 rats per group ). Male rats were fed a basic diet prepared in our Lab., PL-0 (Control ), PL-1, PL-25, PL-50 and PL-75 groups were prepared to be 0%, 1%, 25%, 50% and 75%with distilled water using pyrolig-neous liquor (35% of Choa Co. Ltd.), and were administrated orally for 8 weeks. Superoxide radical contents in liver mi-tochondria and microsomes were significantly decreased to 12-14%, 11-15%, respectively, in these PL-25 and PL-50 groups compared with the control group. Hydroxyl radical content in mitochondria and microsomes were markedly decreased to 12-20% and 17%, respectively, in these PL-25 and PL-50% groups compared with the control group. Hydro-gen peroxide content in mitochondria and microsomes were significantly decreased about 15-12% and 22-20% in liver of PL-25 and PL-50 groups compared with the control group. Mn-SOD and Cu/Zn-SOD activities in liver of PL-25 and PL-50 groups were remarkably increased to 15-25%, 11-16%, respectively, compared with the control group. GPx activities in mitochondria and microsomes were significantly increased in the liver of PL-25 and PL-50 groups compared with the control group. CAT activities in mitochondria and cytosol were significantly increased to 12-14%, 15-27%, respectively, in the liver of PL-25 and PL-50 groups compared with the control group. These results suggest that long term administra-tion orally of 25 and 50% pyroligneous liquor may effectively inhibit the formation of oxygen free radicals, and also scavenger enzyme activities significantly increase through the administration orally.
