Novel Approach Toward the Generation of Tissue Engineered Heart Valve by Using Combination of Antioxidant and Detergent: A Potential Therapy in Cardiovascular Tissue Engineering.
10.1007/s13770-017-0070-1
- Author:
Kishor TARDALKAR
1
;
Shashikant DESAI
;
Arjun ADNAIK
;
Raghvendra BOHARA
;
Meghnad JOSHI
Author Information
1. Department of Stem Cells and Regenerative Medicine, D. Y. Patil University, D. Y. Patil Vidyanagar, Kasba Bawda, Kolhapur, Maharashtra 416006, India. drmeghnadjoshi@gmail.com
- Publication Type:Original Article
- Keywords:
Decellularization;
Heart valve;
Tissue engineering;
Scaffold;
Cardiovascular
- MeSH:
Alcian Blue;
Detergents*;
Dimethyl Sulfoxide;
Extracellular Matrix;
Heart Valves*;
Heart*;
Microscopy, Electron, Scanning;
Regeneration;
Sodium Dodecyl Sulfate;
Tissue Engineering*
- From:
Tissue Engineering and Regenerative Medicine
2017;14(6):755-762
- CountryRepublic of Korea
- Language:English
-
Abstract:
To develop decellularized heart valve scaffold from porcine for heart valve regeneration. Porcine heart valves were decellularized with unique optimized approach by using 1% sodium dodecyl sulfate solution and 5% dimethyl sulfoxide for the first time. Effect of decellularization process on scaffold were characterized by hematoxylin-eosin, 4′,6-diamidino-2-phenylindole, Masson's trichrome, alcian blue staining and scanning electron microscopy for extracellular matrix (ECM) analysis in scaffold. The results showed that developed protocol for decellularization of heart valve scaffold shown complete removal of all cellular components, without changing the properties of ECM. The developed protocol was successfully used for heart valve ECM scaffolds development from porcine. The developed protocol seems to be promising solution for the heart valve tissue engineering application.
