Immunohistochemical Study between Extracellular Matrix Protein Tenascin and T Lymphocytes Distribution in Cholesteatoma.
- Author:
Yong Gi KIM
1
;
Young Gyun PARK
;
Sung Hyun KIM
;
Nam Yong DOH
;
Bong Nam CHOI
;
Doh Young LEE
;
Sung Chul LIM
Author Information
1. Department of Otolaryngology, College of Medicine, Chosun University, Kwangju, Korea. kyg33@chollian.net
- Publication Type:Original Article
- Keywords:
Tenascin;
Cholesteatoma;
Lymphocyte
- MeSH:
Animals;
Antibodies;
B-Lymphocytes;
Biopsy;
Cholesteatoma*;
Ear Canal;
Ear, Middle;
Epithelial Cells;
Extracellular Matrix*;
Lymphocytes;
Mice;
Morphogenesis;
Otitis Media;
Skin;
T-Lymphocytes*;
T-Lymphocytes, Helper-Inducer;
Tenascin*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1998;41(7):839-845
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Components of extracellular matrix protein play a crucial role in morphogenesis and epithelial cell differentiation. Protein is highly conserved during evolution and is expressed in a variety of tissues, and the amount of T and B lymphocytes are significantly higher in cholesteatomatous tissue than in the external auditory canal skin. We examined the spatiotemporal expression and distribution of extracelluar matrix protein tenascin and lymphocytes of cholesteatomatous tissue and normal external auditory canal skin. MATERIAL AND METHODS: The biopsy specimens of cholesteatomatous tissue and external auditory canal skin were obtained during middle ear surgery. Monoclonal mouse antibodies against tenascin, CD4 helper cell and CD8 cytotoxic/suppressor T cells were used. RESULTS: The results are as follows: 1) The expression of tenascin was significantly increased in cholesteatomatous tissue compared to that in normal external auditory canal skin. 2) As the expression of T lymphocytes increased, the expression of tenascin increased significantly (p<0.05, r=0.832). 3) As the expression of Langerhans' cells increased, the expression of tenascin increased significantly (p<0.05, r=0.788). CONCLUSION: These results suggest that the change in the composition of the extracelluar matrix protein and lymphocyte in cholesteatomatous tissue may play an important role in the pathogenesis of cholesteatomatous otitis media.
