Functional Studies of Acyl-CoA Synthetase 4 in the Rat Liver.
- Author:
Young Hee JEONG
1
;
Seung Ju MOON
;
Man Jong KANG
Author Information
1. Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture and Life Science, Biotechnology Research Institute, Chonnam National University, Gwangju, Korea.
- Publication Type:Original Article
- Keywords:
acyl-CoA synthetase;
arachidonic acid;
subcellular fractionation;
northern blotting;
western blotting;
fasting;
high fat diet;
fat-free high sucrose diet;
DEHP[Di-(2-ethylhexyl) phthalatel
- MeSH:
Adult;
Animals;
Arachidonic Acid;
Blotting, Northern;
Blotting, Western;
Diet;
Diet, High-Fat;
Fasting;
Humans;
Insulin;
Ligases*;
Liver*;
Male;
Microsomes;
Mitochondria;
Peroxisomes;
Rats*;
RNA;
RNA, Messenger;
Sucrose
- From:The Korean Journal of Nutrition
2003;36(4):376-381
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues. We examined ACS4 in rat liver, which contains a variety of pathways that use acyl-CoAs, in order to determine subcellular locations. We demonstrate that ACS4 protein was present most abundantly in the mitochondria and to a much lesser extent in the peroxisomes and microsomes. To determined the dietary effects on the level of ACS4 mRNA, northern blotting was carried out using total RNA from the livers of adult male rats fed various diets. Fasting, high fat diet, and fat-free high sucrose diet increased the hepatic level of ACS4 mRNA approximately 2-fold. Furthermore, the levels of ACS4 mRNA were induced by DEHP[Di- (2-ethylhexyl) phthalate]. These data suggest that ACS4 expression in the liver is regulated with a variety of pathways, including beta-oxidation, hormone, and insulin.
