High Concentration of Glucose Induces MUC5B Expression via Reactive Oxygen Species in Human Airway Epithelial Cells.
10.3342/kjorl-hns.2017.17601
- Author:
Young Ha LEE
1
;
Yong Dae KIM
;
Yoo Sun SONG
;
Chang Hoon BAE
;
Yoon Seok CHOI
;
Kyeong Cheol SHIN
;
Hyun Jung JIN
;
Si Youn SONG
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Yeungnam University, Daegu, Korea. ssykhs@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Airway epithelial cell;
Glucose;
MUC5B;
Reactive oxygen species
- MeSH:
Epithelial Cells*;
Flow Cytometry;
Glucose*;
Humans*;
Hyperglycemia;
Immunoenzyme Techniques;
Methods;
Mucins;
NADP;
Oxidoreductases;
Reactive Oxygen Species*;
Real-Time Polymerase Chain Reaction
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2017;60(8):396-403
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The effects of hyperglycemia on the mucin secretion in inflammatory respiratory diseases are not clear. Therefore, this study was conducted to characterize the effect of hyperglycemia, and the mechanism involved, on MUC5AC and MUC5B expression in human airway epithelial cells. SUBJECTS AND METHOD: The NCI-H292 cells and the primary cultures of human nasal epithelial cells were exposed to different concentration of glucose (5, 10, 15, 20, 30 mM) for 8 or 24 hours, the effects of high concentration of glucose (20 mM) on MUC5AC and MUC5B expression were determined using reverse transcriptase-polymerase chain reaction (PCR), real-time PCR and enzyme immunoassay. Measurement of reactive oxygen species (ROS) production was performed by flow cytometry. To investigate the role of ROS in high concentration of glucose-induced MUC5B expression, the cells were pretreated with N-acetyl-cysteine (NAC, 50 mM) as a ROS scavenger, or diphenyleneiodonium (DPI, 100 nM) as a nicotinamide adenine dinucleotide phosphate oxidase inhibitor for 1 hour. RESULTS: In the NCI-H292 cells and the primary cultures of human nasal epithelial cells, High concentration of glucose increased MUC5B expression but did not increase MUC5AC expression (p<0.05). ROS production was also increased by high concentration of glucose (20 mM) (p<0.05). In addition, high concentration of glucose (20 mM)-induced MUC5B expression and ROS production were significantly attenuated by pretreatment of NAC (50 mM) or DPI (100 nM) (p<0.05). CONCLUSION: High concentration of glucose induces MUC5B expressions via ROS in human airway epithelial cells.
