Role of IL-1alpha in Cisplatin-Induced Acute Renal Failure in Mice.
10.3904/kjim.2011.26.2.187
- Author:
Jay Wook LEE
1
;
Woo Jin NAM
;
Min Jee HAN
;
Jung Ho SHIN
;
Jin Gun KIM
;
Su Hyun KIM
;
Hye Ryoun KIM
;
Dong Jin OH
Author Information
1. Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea. intmdoh@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cisplatin;
Interleukin-1alpha;
Acute kidney injury
- MeSH:
Acute Kidney Injury/chemically induced/*immunology/pathology/physiopathology/prevention & control;
Animals;
Antigens, CD11b/analysis;
Apoptosis;
Biological Markers/blood;
Blood Urea Nitrogen;
*Cisplatin;
Creatinine/blood;
Disease Models, Animal;
Fluorescent Antibody Technique;
Integrin alpha2/analysis;
Interleukin-1alpha/deficiency/genetics/*metabolism;
Kidney/*immunology/pathology/physiopathology;
Killer Cells, Natural/immunology;
Macrophages/immunology;
Mice;
Mice, Inbred C57BL;
Mice, Transgenic;
Natural Killer T-Cells/immunology;
Necrosis;
Neutrophil Infiltration;
Time Factors
- From:The Korean Journal of Internal Medicine
2011;26(2):187-194
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: For unknown reasons, caspase-1 -/- mice, protected against cisplatin-induced acute renal failure (ARF), are deficient in interleukin (IL)-1alpha. We thus asked whether IL-1alpha deficiency underlies the mechanism of protection against cisplatin-induced ARF in these mice. METHODS: Cisplatin (30 mg/kg) was injected intraperitoneally into wild-type C57BL/6 mice to produce a cisplatin-induced model of ARF. IL-1alpha was measured in control vehicle- and cisplatin-treated wild-type animals. We also examined whether IL-1alpha -/- mice were similarly protected against cisplatin-induced ARF. Additionally, infiltration of CD11b- and CD49b-positive cells, as markers of macrophages, natural killer, and natural killer T cells (pan-NK cells), was investigated in wild-type and IL-1alpha -/- mice. RESULTS: Compared with vehicle-treated mice, renal IL-1alpha increased in cisplatin-treated wild-type mice beginning on day 1. IL-1alpha -/- mice were shown to be protected against cisplatin-induced ARF. No significant difference in the infiltration of neutrophils or CD11b- and CD49b-positive cells were observed between wild-type and IL-1alpha -/- mice. CONCLUSIONS: Mice deficient in IL-1alpha are protected against cisplatin-induced ARF. The lack of IL-1alpha may explain, at least in part, the protection against cisplatin-induced ARF observed in caspase-1 -/- mice. Investigation of the protective mechanism (s) in IL-1alpha -/- mice in cisplatin-induced ARF merits further study.
