Correlation between Dose of Ethacrynic Acid and Weight of Cat in the Cat Deafening Procedure.
10.3342/kjorl-hns.2010.53.12.749
- Author:
Jeong Hun JANG
1
;
Ho Sun LEE
;
Hye Young KIM
;
Jun Ho LEE
;
Sun O CHANG
;
Seung Ha OH
;
Min Hyun PARK
Author Information
1. Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Ethacrynic acid;
Cochlea;
Deafness;
Aminoglycosides
- MeSH:
Adult;
Aminoglycosides;
Animals;
Body Weight;
Cats;
Cochlea;
Deafness;
Ethacrynic Acid;
Evoked Potentials, Auditory, Brain Stem;
Hearing;
Hearing Loss;
Humans;
Infusions, Intravenous;
Injections, Subcutaneous;
Kanamycin;
Models, Animal;
Organ of Corti;
Organothiophosphorus Compounds;
Spiral Ganglion
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2010;53(12):749-754
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Co-administration of kanamycin (KM) with the loop diuretic ethacrynic acid (EA) has been known to produce a rapid and profound hearing loss in adult animals. The objective of this study was to see if monitoring the hearing status during intravenous infusion of EA could minimalize individual variability and to evaluate the correlation between the dose of EA and the body weight (wt). MATERIALS AND METHOD: Twenty cats with the mean age of 24 weeks+/-3.7 (range, 20.6-28.3 weeks) and the mean weight of 3.27 kg+/-0.75 (range 2.4-4.75 kg) received a subcutaneous injection of KM (300 mg/kg) followed by an intravenous infusion of EA (1 mg/min). Click evoked auditory brainstem responses (ABRs) were recorded to monitor the hearing during the infusion. When the ABR thresholds rose to levels in excess of 90 dB SPL, the infusion of EA was stopped. The histopathologies for sections of apex, middle, base of cochlea were examined after 6 months. RESULTS: There was a significant positive correlation (p<.001, r2=.583) between the EA dose and body weight. Cochlear histopathology showed an absence of organ of Corti and decrease of spiral ganglion cells in the majority of cochleas, especially in the basal turn. The extent of loss of spiral ganglion cells was dependent on their distance from the round window. CONCLUSION: Monitoring the animal's hearing status during the procedure ensured that the dose of EA was optimized for individual animals. Thus, the positive correlation between the EA dose and body weight should be considered should in designing the animal models of controlled high frequency hearing loss.
