A Mouse Model of Chronic Rhinosinusitis Induced by Streptococcus Pneumoniae.
- Author:
Byung Hoon AHN
1
;
Youn Ho PARK
;
Dong Eun KIM
;
In Hyuk SONG
;
Young Jin NAM
;
Sung Il NAM
;
Sang Pyo KIM
;
Nam Hee RYOO
Author Information
1. Department of Otolaryngology, School of Medicine, Keimyung University, Daegu, Korea. bhahn@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Sinusitis;
Mice;
Streptococcus pneumoniae
- MeSH:
Animals;
Bacteria;
Eosine Yellowish-(YS);
Genetics;
Goblet Cells;
Hematoxylin;
Humans;
Hyperplasia;
Male;
Maxillary Sinus;
Mice*;
Mucous Membrane;
Pneumonia;
Septum of Brain;
Sinusitis;
Stem Cells;
Streptococcus pneumoniae*;
Streptococcus*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2006;49(1):52-59
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: A mouse has a great potential to be used in studying genetics and inflammatory process of the rhinosinusitis. The aim of this study was to observe effects of experimentally induced chronic rhinosinusitis on histopathology of the sinonasal mucosa in a mouse and to develop a chronic form of rhinosinusitis. MATERIALS AND METHOD: Thirty five, six-week old male C57BL/6 mice were used as follows: 7 normal controls without intervention, 7 Sham operated controls, 7 animals with ostial obstruction alone using Merocel, 7 animals implanted with Merocel plus 106 colony forming unit (CFU)/mL of Streptococcus pneumoniae and 7 animals implanted with Merocel plus 10(8) CFU/mL of S. pneumoniae. Six weeks after intervention, the animals were sacrificed and serially sectioned at 1 mm intervals and stained with Hematoxylin and Eosin. RESULTS: Increased epithelial thickness, goblet cell hyperplasia, epithelial disarray and inflammatory infiltration were observed in the experimental sinuses packed with Merocel alone or Merocel with bacterial inoculation, especially at the nasal septal area. However, there were no significant differences between the Merocel only inserted group and Merocel and bacteria inoculated group. CONCLUSION: Maxillary sinus ostial obstruction or ostial obstruction with S. pneumoniae inoculation induced chronic rhinosinusitis in C57BL/6 mice as indicated by the histologic change. This study could be used as a model of chronic rhinosinusitis for further study.
